biostudies-literatureUnknown15Benjamaa RThis study assessed the medicinal properties of <i>Euphorbia resinifera O. Berg (E. resinifera)</i> and <i>Euphorbia officinarum subsp echinus (Hook.f. and Coss.) Vindt</i> (<i>Euphorbia echinus</i>, known for their pharmaceutical benefits. Extracts from their flowers, stems, propolis, and honey were examined for phenolic content, antioxidant, anti-inflammatory, and antibacterial activities. Total phenolic content (TPC), total flavonoid content (TFC), and total condensed tannin (TCC) were determined using specific methods. Antioxidant potential was assessed through various tests including DPPH, FRAP, ABTS, and Total antioxidant capacity. Anti-inflammatory effects were evaluated using phenol-induced ear edema in rats, while antibacterial activity was measured against Gram-positive (<i>Staphylococcus aureus</i> ATCC 6538) and Gram-negative (<i>E. coli</i> ATCC 10536) bacteria. Among the extracts, the aqueous propolis extract of <i>E. resinifera</i> demonstrated exceptional antioxidant capabilities, with low IC₅₀ values for DPPH (0.07 ± 0.00 mg/mL) and ABTS (0.13 ± 0.00 mg/mL), as well as high TAC (176.72 ± 0.18 mg AA/mg extract) and FRAP (86.45 ± 1.45 mg AA/mg extract) values. Furthermore, the anti-inflammatory effect of <i>E. resinifera</i> propolis extracts surpassed that of indomethacin, yielding edema percentages of 3.92% and 11.33% for the aqueous and ethanolic extracts, respectively. Microbiological results indicated that the aqueous extract of <i>E. resinifera</i> flower exhibited the most potent inhibitory action against <i>S. aureus</i>, with an inhibition zone diameter (IZD) of 21.0 ± 0.00 mm and a minimum inhibitory concentration (MIC) of 3.125 mg/mL. Additionally, only <i>E. resinifera</i> honey displayed the ability to inhibit <i>E. coli</i> growth, with an inhibition zone diameter of 09.30 ± 0.03 mm and a MIC of 0.0433 mg/mL.Frontiers in pharmacology1345340https://www.ebi.ac.uk/biostudies/studies/S-EPMC10919402biostudies-literatureComparative evaluation of antioxidant activity, total phenolic content, anti-inflammatory, and antibacterial potential of <i>Euphorbia</i>-derived functional products.PMC10919402Gupta RChoi EHEnnibi OErrati HBenjamaa RElbouny HEssamadi AMoujanni AKaushik NNasser BKaushik NKfalseComparative evaluation of antioxidant activity, total phenolic content, anti-inflammatory, and antibacterial potential of <i>Euphorbia</i>-derived functional products.This study assessed the medicinal properties of <i>Euphorbia resinifera O. Berg (E. resinifera)</i> and <i>Euphorbia officinarum subsp echinus (Hook.f. and Coss.) Vindt</i> (<i>Euphorbia echinus</i>, known for their pharmaceutical benefits. Extracts from their flowers, stems, propolis, and honey were examined for phenolic content, antioxidant, anti-inflammatory, and antibacterial activities. Total phenolic content (TPC), total flavonoid content (TFC), and total condensed tannin (TCC) were determined using specific methods. Antioxidant potential was assessed through various tests including DPPH, FRAP, ABTS, and Total antioxidant capacity. Anti-inflammatory effects were evaluated using phenol-induced ear edema in rats, while antibacterial activity was measured against Gram-positive (<i>Staphylococcus aureus</i> ATCC 6538) and Gram-negative (<i>E. coli</i> ATCC 10536) bacteria. Among the extracts, the aqueous propolis extract of <i>E. resinifera</i> demonstrated exceptional antioxidant capabilities, with low IC₅₀ values for DPPH (0.07 ± 0.00 mg/mL) and ABTS (0.13 ± 0.00 mg/mL), as well as high TAC (176.72 ± 0.18 mg AA/mg extract) and FRAP (86.45 ± 1.45 mg AA/mg extract) values. Furthermore, the anti-inflammatory effect of <i>E. resinifera</i> propolis extracts surpassed that of indomethacin, yielding edema percentages of 3.92% and 11.33% for the aqueous and ethanolic extracts, respectively. Microbiological results indicated that the aqueous extract of <i>E. resinifera</i> flower exhibited the most potent inhibitory action against <i>S. aureus</i>, with an inhibition zone diameter (IZD) of 21.0 ± 0.00 mm and a minimum inhibitory concentration (MIC) of 3.125 mg/mL. Additionally, only <i>E. resinifera</i> honey displayed the ability to inhibit <i>E. coli</i> growth, with an inhibition zone diameter of 09.30 ± 0.03 mm and a MIC of 0.0433 mg/mL.2024-01-01T00:00:00Z20242026-06-28T03:17:33.548Z2025-04-04T12:34:35.629ZS-EPMC109194023845595810.3389/fphar.2024.1345340