biostudies-literatureSalas-Gallardo GAConsejo Nacional de Ciencia y TecnologíaDirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de Méxicoe0297576https://www.ebi.ac.uk/biostudies/studies/S-EPMC10919594biostudies-literatureUnknown19(3)The Peritumoral Brain Zone (PBZ) contributes to Glioblastoma (GBM) relapse months after the resection of the original tumor, which is influenced by a variety of pathological factors. Among those, microglia are recognized as one of the main regulators of GBM progression and probably relapse. Although microglial morphology has been analyzed inside GBM and its immediate surroundings, it has not been objectively characterized throughout the PBZ. Thus, we aimed to perform a thorough characterization of microglial morphology in the PBZ and its likely differentiation not just from the tumor-associated microglia but from control tissue microglia. For this purpose, Sprague Dawley rats were intrastriatally implanted with C6 cells to induce a GBM formation. Gadolinium-based magnetic resonance imagingPloS oneMorphological differentiation of peritumoral brain zone microglia.PMC10919594A1-S-7540IG200521Pena-Ortega FRobles-Gomez AASalas-Gallardo GALorea-Hernandez JJDel Campo CCfalseMorphological differentiation of peritumoral brain zone microglia.The Peritumoral Brain Zone (PBZ) contributes to Glioblastoma (GBM) relapse months after the resection of the original tumor, which is influenced by a variety of pathological factors. Among those, microglia are recognized as one of the main regulators of GBM progression and probably relapse. Although microglial morphology has been analyzed inside GBM and its immediate surroundings, it has not been objectively characterized throughout the PBZ. Thus, we aimed to perform a thorough characterization of microglial morphology in the PBZ and its likely differentiation not just from the tumor-associated microglia but from control tissue microglia. For this purpose, Sprague Dawley rats were intrastriatally implanted with C6 cells to induce a GBM formation. Gadolinium-based magnetic resonance imaging2024-01-01T00:00:00Z20242026-06-12T10:10:08.706Z2025-04-04T12:34:55.537ZS-EPMC109195943845195810.1371/journal.pone.0297576