{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["El-Ayoubi A"],"funding":["Robert Bosch Stiftung Stuttgart","DFG Exzellenzstrategie des Bundes und der Länder","Deutsche Krebshilfe"],"pagination":["528-546"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10920084"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(3)"],"pubmed_abstract":["Neural stem cells (NSCs) are considered to be valuable candidates for delivering a variety of anti-cancer agents, including oncolytic viruses, to brain tumors. However, owing to the previously reported tumorigenic potential of NSC cell lines after intranasal administration (INA), here we identified the human hepatic stellate cell line LX-2 as a cell type capable of longer resistance to replication of oncolytic adenoviruses (OAVs) as a therapeutic cargo, and that is non-tumorigenic after INA. Our data show that LX-2 cells can longer withstand the OAV XVir-N-31 replication and oncolysis than NSCs. By selecting the highly migratory cell population out of LX-2, an offspring cell line with a higher and more stable capability to migrate was generated. Additionally, as a safety backup, we applied"],"journal":["Molecular oncology"],"pubmed_title":["Development of an optimized, non-stem cell line for intranasal delivery of therapeutic cargo to the central nervous system."],"pmcid":["PMC10920084"],"funding_grant_id":["70113907","EXC 2180-390900677"],"pubmed_authors":["Mikulits W","Schwab M","El-Ayoubi A","Klawitter M","Merk L","Gonzalez-Menendez I","Naumann U","Holm PS","Quintanilla Fend L","Arakelyan A","Hakobyan S","Danielyan L"],"additional_accession":[]},"is_claimable":false,"name":"Development of an optimized, non-stem cell line for intranasal delivery of therapeutic cargo to the central nervous system.","description":"Neural stem cells (NSCs) are considered to be valuable candidates for delivering a variety of anti-cancer agents, including oncolytic viruses, to brain tumors. However, owing to the previously reported tumorigenic potential of NSC cell lines after intranasal administration (INA), here we identified the human hepatic stellate cell line LX-2 as a cell type capable of longer resistance to replication of oncolytic adenoviruses (OAVs) as a therapeutic cargo, and that is non-tumorigenic after INA. Our data show that LX-2 cells can longer withstand the OAV XVir-N-31 replication and oncolysis than NSCs. By selecting the highly migratory cell population out of LX-2, an offspring cell line with a higher and more stable capability to migrate was generated. Additionally, as a safety backup, we applied","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-30T03:35:50.186Z","creation":"2026-06-30T03:33:12.086Z"},"accession":"S-EPMC10920084","cross_references":{"pubmed":["38115217"],"doi":["10.1002/1878-0261.13569"]}}