biostudies-literatureDuarte ABSCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorFundação de Apoio à Pesquisa do Estado da Paraíba25-39https://www.ebi.ac.uk/biostudies/studies/S-EPMC10920570biostudies-literatureUnknown55(1)The objective of this study was to evaluate the antifungal activity of free methyl 3,5 dinitrobenzoate (MDNB) and its nanoemulsion (MDNB-NE) against strains of Candida albicans. Additionally, a molecular modeling study was also carried out to propose the mechanism of action and toxicity of MDNB. These results demonstrated the MDNB-NE presented a droplet size of 181.16 ± 3.20 nm and polydispersity index of 0.30 ± 0.03. MDNB and MDNB-NE inhibited the growth of all strains with minimum inhibitory concentrations of 0.27-1.10 mM. The biological results corroborated the molecular model, which pointed to a multi-target antifungal mechanism of action for MDNB in C. albicans. The study could serve as a basis for further research involving compounds with nitro groups with antifungal.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Antifungal activity against Candida albicans of methyl 3,5-dinitrobenzoate loaded nanoemulsion.PMC10920570001Perez-Castillo YOliveira EEde Souza RLDuarte ABSde Castro RDde Sousa DPda Nobrega Alves DfalseAntifungal activity against Candida albicans of methyl 3,5-dinitrobenzoate loaded nanoemulsion.The objective of this study was to evaluate the antifungal activity of free methyl 3,5 dinitrobenzoate (MDNB) and its nanoemulsion (MDNB-NE) against strains of Candida albicans. Additionally, a molecular modeling study was also carried out to propose the mechanism of action and toxicity of MDNB. These results demonstrated the MDNB-NE presented a droplet size of 181.16 ± 3.20 nm and polydispersity index of 0.30 ± 0.03. MDNB and MDNB-NE inhibited the growth of all strains with minimum inhibitory concentrations of 0.27-1.10 mM. The biological results corroborated the molecular model, which pointed to a multi-target antifungal mechanism of action for MDNB in C. albicans. The study could serve as a basis for further research involving compounds with nitro groups with antifungal.2024-01-01T00:00:00Z2024 Mar2026-07-02T03:21:10.543Z2025-04-07T00:26:58.736ZS-EPMC109205703813580510.1007/s42770-023-01214-9