{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tsukalov I"],"funding":["Ministry of Economy and Competitiveness | Instituto de Salud Carlos III (Institute of Health Carlos III)","Comunidad de Madrid","Ministry of Economy and Competitiveness | Instituto de Salud Carlos III","Ministerio de Economía y Competitividad (Ministry of Economy and Competitiveness)","Ministerio de Economía y Competitividad","Ministry of Economy and Competitiveness | Agencia Estatal de Investigación","Ministry of Economy and Competitiveness | Agencia Estatal de Investigación (Spanish Agencia Estatal de Investigación)"],"pagination":["2100"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10920883"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Increased recruitment of transitional and non-classical monocytes in the lung during SARS-CoV-2 infection is associated with COVID-19 severity. However, whether specific innate sensors mediate the activation or differentiation of monocytes in response to different SARS-CoV-2 proteins remain poorly characterized. Here, we show that SARS-CoV-2 Spike 1 but not nucleoprotein induce differentiation of monocytes into transitional or non-classical subsets from both peripheral blood and COVID-19 bronchoalveolar lavage samples in a NFκB-dependent manner, but this process does not require inflammasome activation. However, NLRP3 and NLRC4 differentially regulated CD86 expression in monocytes in response to Spike 1 and Nucleoprotein, respectively. Moreover, monocytes exposed to Spike 1 induce significantly higher proportions of Th1 and Th17 CD4 + T cells. In contrast, monocytes exposed to Nucleoprotein reduce the degranulation of CD8 + T cells from severe COVID-19 patients. Our study provides insights in the differential impact of innate sensors in regulating monocytes in response to different SARS-CoV-2 proteins, which might be useful to better understand COVID-19 immunopathology and identify therapeutic targets."],"journal":["Nature communications"],"pubmed_title":["NFκB and NLRP3/NLRC4 inflammasomes regulate differentiation, activation and functional properties of monocytes in response to distinct SARS-CoV-2 proteins."],"pmcid":["PMC10920883"],"funding_grant_id":["INMUNOVACTER REACT-EU","CIBERINFECC","PID-2020-120412RB-I00","Inmunovacter REACT-UE","PID2021-127899OB-I00","CIBERCV","PDC2021-121719-I00"],"pubmed_authors":["Esparcia L","Gonzalez Alvaro I","de Los Santos I","Calvet-Mirabent M","Alfranca A","Iturricastillo G","Martinez-Fleta P","Marcos-Jimenez A","Sanchez-Cerrillo I","Ancochea J","Scagnetti C","Sanchez-Madrid F","Genesca M","Martin-Cofreces N","Buzon MJ","Rajas O","Tsukalov I","Calzada MJ","Popova O","Munoz-Calleja C","Delgado-Arevalo C","Martin-Gayo E","Avalos E","Palacios-Calvo J"],"additional_accession":[]},"is_claimable":false,"name":"NFκB and NLRP3/NLRC4 inflammasomes regulate differentiation, activation and functional properties of monocytes in response to distinct SARS-CoV-2 proteins.","description":"Increased recruitment of transitional and non-classical monocytes in the lung during SARS-CoV-2 infection is associated with COVID-19 severity. However, whether specific innate sensors mediate the activation or differentiation of monocytes in response to different SARS-CoV-2 proteins remain poorly characterized. Here, we show that SARS-CoV-2 Spike 1 but not nucleoprotein induce differentiation of monocytes into transitional or non-classical subsets from both peripheral blood and COVID-19 bronchoalveolar lavage samples in a NFκB-dependent manner, but this process does not require inflammasome activation. However, NLRP3 and NLRC4 differentially regulated CD86 expression in monocytes in response to Spike 1 and Nucleoprotein, respectively. Moreover, monocytes exposed to Spike 1 induce significantly higher proportions of Th1 and Th17 CD4 + T cells. In contrast, monocytes exposed to Nucleoprotein reduce the degranulation of CD8 + T cells from severe COVID-19 patients. Our study provides insights in the differential impact of innate sensors in regulating monocytes in response to different SARS-CoV-2 proteins, which might be useful to better understand COVID-19 immunopathology and identify therapeutic targets.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-27T03:17:14.639Z","creation":"2026-06-27T03:06:48.315Z"},"accession":"S-EPMC10920883","cross_references":{"pubmed":["38453949"],"doi":["10.1038/s41467-024-46322-8"]}}