{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dumelie JG"],"funding":["U.S. Department of Health & Human Services | NIH | Office of Extramural Research, National Institutes of Health","NICHD NIH HHS","NIEHS NIH HHS","NINDS NIH HHS","NCI NIH HHS","NIAMS NIH HHS","U.S. Department of Health & Human Services | National Institutes of Health","NIH HHS"],"pagination":["302-313"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10922641"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(3)"],"pubmed_abstract":["Proteins and RNA can phase separate from the aqueous cellular environment to form subcellular compartments called condensates. This process results in a protein-RNA mixture that is chemically different from the surrounding aqueous phase. Here, we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and extracts of cellular metabolites and identified metabolites enriched in the condensate phase. Among the most condensate-enriched metabolites were phospholipids, due primarily to the hydrophobicity of their fatty acyl moieties. We found that phospholipids can alter the number and size of phase-separated condensates and in some cases alter their morphology. Finally, we found that phospholipids partition into a diverse set of endogenous condensates as well as artificial condensates expressed in cells. Overall, these data show that many condensates are protein-RNA-lipid mixtures with chemical microenvironments that are ideally suited to facilitate phospholipid biology and signaling."],"journal":["Nature chemical biology"],"pubmed_title":["Biomolecular condensates create phospholipid-enriched microenvironments."],"pmcid":["PMC10922641"],"funding_grant_id":["R21ES032347","R01 CA186702","R35 NS111631","R01CA186702","P01 HD067244","R21 NS118233","R21 ES032347","R01AR076029","R35NS111631","R21NS118633","S10 OD030335","R01 AR076029"],"pubmed_authors":["Dumelie JG","Gross SS","Chen Q","Attarwala N","Miller D","Jaffrey SR"],"additional_accession":[]},"is_claimable":false,"name":"Biomolecular condensates create phospholipid-enriched microenvironments.","description":"Proteins and RNA can phase separate from the aqueous cellular environment to form subcellular compartments called condensates. This process results in a protein-RNA mixture that is chemically different from the surrounding aqueous phase. Here, we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and extracts of cellular metabolites and identified metabolites enriched in the condensate phase. Among the most condensate-enriched metabolites were phospholipids, due primarily to the hydrophobicity of their fatty acyl moieties. We found that phospholipids can alter the number and size of phase-separated condensates and in some cases alter their morphology. Finally, we found that phospholipids partition into a diverse set of endogenous condensates as well as artificial condensates expressed in cells. Overall, these data show that many condensates are protein-RNA-lipid mixtures with chemical microenvironments that are ideally suited to facilitate phospholipid biology and signaling.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-02T19:15:43.037Z","creation":"2025-04-04T02:47:50.253Z"},"accession":"S-EPMC10922641","cross_references":{"pubmed":["37973889"],"doi":["10.1038/s41589-023-01474-4"]}}