{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sutton HJ"],"funding":["National Institute of Allergy and Infectious Diseases","Intramural NIH HHS","National Health and Medical Research Council","National Institutes of Health","Division of Intramural Research"],"pagination":["245-255.e5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10922795"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["57(2)"],"pubmed_abstract":["Long-lived plasma cells (PCs) secrete antibodies that can provide sustained immunity against infection. High-affinity cells are proposed to preferentially select into this compartment, potentiating the immune response. We used single-cell RNA-seq to track the germinal center (GC) development of Igh<sup>g2A10</sup> B cells, specific for the Plasmodium falciparum circumsporozoite protein (PfCSP). Following immunization with Plasmodium sporozoites, we identified 3 populations of cells in the GC light zone (LZ). One LZ population expressed a gene signature associated with the initiation of PC differentiation and readily formed PCs in vitro. The estimated affinity of these pre-PC B cells was indistinguishable from that of LZ cells that remained in the GC. This remained true when high- or low-avidity recombinant PfCSP proteins were used as immunogens. These findings suggest that the initiation of PC development occurs via an affinity-independent process."],"journal":["Immunity"],"pubmed_title":["Lack of affinity signature for germinal center cells that have initiated plasma cell differentiation."],"pmcid":["PMC10922795"],"funding_grant_id":["GNT2003035","GNT1158404","ZIA AI001260","GNT2008648"],"pubmed_authors":["Kelly HG","Parker BJ","Tan J","Neeman T","Gao X","Sutton HJ","Lofgren M","Dacon C","Chatterjee D","Seder RA","Idris AH","Cockburn IA"],"additional_accession":[]},"is_claimable":false,"name":"Lack of affinity signature for germinal center cells that have initiated plasma cell differentiation.","description":"Long-lived plasma cells (PCs) secrete antibodies that can provide sustained immunity against infection. High-affinity cells are proposed to preferentially select into this compartment, potentiating the immune response. We used single-cell RNA-seq to track the germinal center (GC) development of Igh<sup>g2A10</sup> B cells, specific for the Plasmodium falciparum circumsporozoite protein (PfCSP). Following immunization with Plasmodium sporozoites, we identified 3 populations of cells in the GC light zone (LZ). One LZ population expressed a gene signature associated with the initiation of PC differentiation and readily formed PCs in vitro. The estimated affinity of these pre-PC B cells was indistinguishable from that of LZ cells that remained in the GC. This remained true when high- or low-avidity recombinant PfCSP proteins were used as immunogens. These findings suggest that the initiation of PC development occurs via an affinity-independent process.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Feb","modification":"2025-04-04T02:01:03.603Z","creation":"2025-04-04T02:01:03.603Z"},"accession":"S-EPMC10922795","cross_references":{"pubmed":["38228150"],"doi":["10.1016/j.immuni.2023.12.010"]}}