{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Vo Q"],"funding":["NHLBI NIH HHS"],"pagination":["2304630"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10923530"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["34(10)"],"pubmed_abstract":["Preclinical human-relevant modeling of organ-specific vasculature offers a unique opportunity to recreate pathophysiological intercellular, tissue-tissue, and cell-matrix interactions for a broad range of applications. Here, we present a reliable, and simply reproducible process for constructing user-controlled long rounded extracellular matrix (ECM)-embedded vascular microlumens on-chip for endothelization and co-culture with stromal cells obtained from human lung. We demonstrate the critical impact of microchannel cross-sectional geometry and length on uniform distribution and magnitude of vascular wall shear stress, which is key when emulating <i>in vivo</i>-observed blood flow biomechanics in health and disease. In addition, we provide an optimization protocol for multicellular culture"],"journal":["Advanced functional materials"],"pubmed_title":["On-Chip Reconstitution of Uniformly Shear-Sensing 3D Matrix-embedded Multicellular Blood Microvessel."],"pmcid":["PMC10923530"],"funding_grant_id":["R01 HL159494"],"pubmed_authors":["Vo Q","Ajiboye AS","Carlson KA","Benam KH","Wier EM","Chiknas PM","Brocker CN","DaSilva L","Park SK","Clark E"],"additional_accession":[]},"is_claimable":false,"name":"On-Chip Reconstitution of Uniformly Shear-Sensing 3D Matrix-embedded Multicellular Blood Microvessel.","description":"Preclinical human-relevant modeling of organ-specific vasculature offers a unique opportunity to recreate pathophysiological intercellular, tissue-tissue, and cell-matrix interactions for a broad range of applications. Here, we present a reliable, and simply reproducible process for constructing user-controlled long rounded extracellular matrix (ECM)-embedded vascular microlumens on-chip for endothelization and co-culture with stromal cells obtained from human lung. We demonstrate the critical impact of microchannel cross-sectional geometry and length on uniform distribution and magnitude of vascular wall shear stress, which is key when emulating <i>in vivo</i>-observed blood flow biomechanics in health and disease. In addition, we provide an optimization protocol for multicellular culture","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-18T12:54:58.058Z","creation":"2025-04-06T22:17:02.039Z"},"accession":"S-EPMC10923530","cross_references":{"pubmed":["38465199"],"doi":["10.1002/adfm.202304630"]}}