{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Link N"],"funding":["National Institute of Allergy and Infectious Diseases","Cullen Foundation","NICHD NIH HHS","NIAID NIH HHS","University of Utah","Caroline Wiess Law Fund for Research in Molecular Medicine","Baylor College of Medicine","Naman Family Fund for Basic Research","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["dmm050297"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10924231"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(2)"],"pubmed_abstract":["In the past decade, Zika virus (ZIKV) emerged as a global public health concern. Although adult infections are typically mild, maternal infection can lead to adverse fetal outcomes. Understanding how ZIKV proteins disrupt development can provide insights into the molecular mechanisms of disease caused by this virus, which includes microcephaly. In this study, we generated a toolkit to ectopically express ZIKV proteins in vivo in Drosophila melanogaster in a tissue-specific manner using the GAL4/UAS system. We used this toolkit to identify phenotypes and potential host pathways targeted by the virus. Our work identified that expression of most ZIKV proteins caused scorable phenotypes, such as overall lethality, gross morphological defects, reduced brain size and neuronal function defects. W"],"journal":["Disease models & mechanisms"],"pubmed_title":["A Zika virus protein expression screen in Drosophila to investigate targeted host pathways during development."],"pmcid":["PMC10924231"],"funding_grant_id":["R25 GM069234","R01AI170857","R01 AI170857","P50 HD103555","R56AI170857","R25GM069234","U54 HD083092","2T32HD007491-26A1","T32 HD007491","R56 AI170857"],"pubmed_authors":["Harnish JM","Gibson S","Medina-Balcazar S","Link N","Shah PS","Mgbike UE","Yamamoto S","Hull B","Dietze M"],"additional_accession":[]},"is_claimable":false,"name":"A Zika virus protein expression screen in Drosophila to investigate targeted host pathways during development.","description":"In the past decade, Zika virus (ZIKV) emerged as a global public health concern. Although adult infections are typically mild, maternal infection can lead to adverse fetal outcomes. Understanding how ZIKV proteins disrupt development can provide insights into the molecular mechanisms of disease caused by this virus, which includes microcephaly. In this study, we generated a toolkit to ectopically express ZIKV proteins in vivo in Drosophila melanogaster in a tissue-specific manner using the GAL4/UAS system. We used this toolkit to identify phenotypes and potential host pathways targeted by the virus. Our work identified that expression of most ZIKV proteins caused scorable phenotypes, such as overall lethality, gross morphological defects, reduced brain size and neuronal function defects. W","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Feb","modification":"2026-07-15T13:13:12.913Z","creation":"2026-07-05T03:08:11.895Z"},"accession":"S-EPMC10924231","cross_references":{"pubmed":["38214058"],"doi":["10.1242/dmm.050297"]}}