{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["170(2)"],"submitter":["Chatterjee D"],"funding":["Albert David Limited","Department of Biotechnology, Ministry of Science and Technology, India"],"pubmed_abstract":["Metal homeostasis is maintained by the uptake, storage and efflux of metal ions that are necessary for the survival of the bacterium. Homeostasis is mostly regulated by a group of transporters categorized as ABC transporters and P-type ATPases. On the other hand, efflux pumps often play a role in drug-metal cross-resistance. Here, with the help of antibiotic sensitivity, antibiotic/dye accumulation and semi-quantitative biofilm formation assessments we report the ability of Rv3270, a P-type ATPase known for its role in combating Mn<sup>2+</sup> and Zn<sup>2+</sup> metal ion toxicity in <i>Mycobacterium tuberculosis</i>, in influencing the extrusion of multiple structurally unrelated drugs and enhancing the biofilm formation of <i>Escherichia coli</i> and <i>Mycobacterium smegmatis.</i> Overexpression of Rv3270 increased the tolerance of host cells to norfloxacin, ofloxacin, sparfloxacin, ampicillin, oxacillin, amikacin and isoniazid. A significantly lower accumulation of norfloxacin, ethidium bromide, bocillin FL and levofloxacin in cells harbouring Rv3270 as compared to host cells indicated its role in enhancing efflux activity. Although over-expression of Rv3270 did not alter the susceptibility levels of levofloxacin, rifampicin and apramycin, the presence of a sub-inhibitory concentration of Zn<sup>2+</sup> resulted in low-level tolerance towards these drugs. Of note, the expression of Rv3270 enhanced the biofilm-forming ability of the host cells strengthening its role in antimicrobial resistance. Therefore, the study indicated that the over-expression of Rv3270 enhances the drug efflux activity of the micro-organism where zinc might facilitate drug-metal cross-resistance for some antibiotics."],"journal":["Microbiology (Reading, England)"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10924464"],"repository":["biostudies-literature"],"pubmed_title":["P-type ATPase zinc transporter Rv3270 of <i>Mycobacterium tuberculosis</i> enhances multi-drug efflux activity."],"pmcid":["PMC10924464"],"funding_grant_id":["TN: AP: 204","BT/PR40383/BCE/8/1561/2020"],"pubmed_authors":["Daya Manasi AR","Ghosh AS","Panda AP","Chatterjee D"],"additional_accession":[]},"is_claimable":false,"name":"P-type ATPase zinc transporter Rv3270 of <i>Mycobacterium tuberculosis</i> enhances multi-drug efflux activity.","description":"Metal homeostasis is maintained by the uptake, storage and efflux of metal ions that are necessary for the survival of the bacterium. Homeostasis is mostly regulated by a group of transporters categorized as ABC transporters and P-type ATPases. On the other hand, efflux pumps often play a role in drug-metal cross-resistance. Here, with the help of antibiotic sensitivity, antibiotic/dye accumulation and semi-quantitative biofilm formation assessments we report the ability of Rv3270, a P-type ATPase known for its role in combating Mn<sup>2+</sup> and Zn<sup>2+</sup> metal ion toxicity in <i>Mycobacterium tuberculosis</i>, in influencing the extrusion of multiple structurally unrelated drugs and enhancing the biofilm formation of <i>Escherichia coli</i> and <i>Mycobacterium smegmatis.</i> Overexpression of Rv3270 increased the tolerance of host cells to norfloxacin, ofloxacin, sparfloxacin, ampicillin, oxacillin, amikacin and isoniazid. A significantly lower accumulation of norfloxacin, ethidium bromide, bocillin FL and levofloxacin in cells harbouring Rv3270 as compared to host cells indicated its role in enhancing efflux activity. Although over-expression of Rv3270 did not alter the susceptibility levels of levofloxacin, rifampicin and apramycin, the presence of a sub-inhibitory concentration of Zn<sup>2+</sup> resulted in low-level tolerance towards these drugs. Of note, the expression of Rv3270 enhanced the biofilm-forming ability of the host cells strengthening its role in antimicrobial resistance. Therefore, the study indicated that the over-expression of Rv3270 enhances the drug efflux activity of the micro-organism where zinc might facilitate drug-metal cross-resistance for some antibiotics.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Feb","modification":"2025-04-22T01:02:38.698Z","creation":"2025-04-05T19:49:20.372Z"},"accession":"S-EPMC10924464","cross_references":{"pubmed":["38373028"],"doi":["10.1099/mic.0.001441"]}}