biostudies-literatureShang SSupported by the Research Program of Wuhan Municipal Health and Family Planning Commission3000605241233166https://www.ebi.ac.uk/biostudies/studies/S-EPMC10924567biostudies-literatureUnknown52(3)<h4>Objective</h4>To investigate the correlations between multigene alterations and clinicopathological features in papillary thyroid carcinoma (PTC) samples.<h4>Methods</h4>In this retrospective study, 111 cytological specimens of thyroid nodules, including 74 PTC samples and 37 benign samples, were analyzed using a 22-gene mutation assay employing next-generation sequencing. Clinicopathological information was retrospectively collected and analyzed.<h4>Results</h4>Gene alterations were associated with a higher rate of lymph node metastasis (LNM) and thyroid capsular invasion, a lower rate of coexisting Hashimoto's thyroiditis, the classical PTC subtype, and younger age (<45 years). Among the 22 genes tested, the <i>BRAF</i> mutation rates showed a significant difference between the PTC and benign groups. In the subgroup analysis, younger age (odds ratio = 12.512, 95% confidence interval: 3.126-50.087) was an independent risk factor for LNM. In further analyses, <i>BRAF</i> mutation was significantly associated with LNM in the older subgroup (age ≥ 45 years), suggesting that the <i>BRAF</i> mutation test has greater value for determining PTC prognosis in the older age group.<h4>Conclusions</h4>Our findings will provide a more comprehensive understanding of the relationship between gene mutations and PTC and may contribute to improved PTC management.The Journal of international medical researchCorrelation between genetic alterations and clinicopathological features of papillary thyroid carcinomas.PMC10924567[WH21Z40]Yang HFeng NLiu HWu JLi XZheng ZChen MZhang YShi XShang SfalseCorrelation between genetic alterations and clinicopathological features of papillary thyroid carcinomas.<h4>Objective</h4>To investigate the correlations between multigene alterations and clinicopathological features in papillary thyroid carcinoma (PTC) samples.<h4>Methods</h4>In this retrospective study, 111 cytological specimens of thyroid nodules, including 74 PTC samples and 37 benign samples, were analyzed using a 22-gene mutation assay employing next-generation sequencing. Clinicopathological information was retrospectively collected and analyzed.<h4>Results</h4>Gene alterations were associated with a higher rate of lymph node metastasis (LNM) and thyroid capsular invasion, a lower rate of coexisting Hashimoto's thyroiditis, the classical PTC subtype, and younger age (<45 years). Among the 22 genes tested, the <i>BRAF</i> mutation rates showed a significant difference between the PTC and benign groups. In the subgroup analysis, younger age (odds ratio = 12.512, 95% confidence interval: 3.126-50.087) was an independent risk factor for LNM. In further analyses, <i>BRAF</i> mutation was significantly associated with LNM in the older subgroup (age ≥ 45 years), suggesting that the <i>BRAF</i> mutation test has greater value for determining PTC prognosis in the older age group.<h4>Conclusions</h4>Our findings will provide a more comprehensive understanding of the relationship between gene mutations and PTC and may contribute to improved PTC management.2024-01-01T00:00:00Z2024 Mar2026-06-23T03:20:39.746Z2025-04-22T00:57:13.769ZS-EPMC109245673845665010.1177/03000605241233166