{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Chen SY"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Ninjurin-1 (NINJ1), initially identified as a stress-induced protein in neurons, recently emerged as a key mediator of plasma membrane rupture during apoptosis, necrosis, and pyroptosis. However, its involvement in ferroptosis remains unknown. Here, we demonstrate that NINJ1 also plays a crucial role in ferroptosis, but through a distinct mechanism. NINJ1 knockdown significantly protected cancer cells against ferroptosis induced by xCT inhibitors but no other classes of ferroptosis-inducing compounds (FINs). Glycine, known to inhibit canonical NINJ1-mediated membrane rupture in other cell deaths, had no impact on ferroptosis. A compound screen revealed that NINJ1-mediated ferroptosis protection can be abolished by pantothenate kinase inhibitor (PANKi), buthionine sulfoximine (BSO), and die"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.02.22.581432"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10925083"],"repository":["biostudies-literature"],"pubmed_title":["NINJ1 regulates ferroptosis via xCT antiporter interaction and CoA modulation."],"pmcid":["PMC10925083"],"funding_grant_id":["R01 GM124062","R21 AI149205"],"pubmed_authors":["Wu J","Setayeshpour Y","Chen SY","Lin CC","Wang YE","Mestre A","Chi JT","Chen Y"],"additional_accession":[]},"is_claimable":false,"name":"NINJ1 regulates ferroptosis via xCT antiporter interaction and CoA modulation.","description":"Ninjurin-1 (NINJ1), initially identified as a stress-induced protein in neurons, recently emerged as a key mediator of plasma membrane rupture during apoptosis, necrosis, and pyroptosis. However, its involvement in ferroptosis remains unknown. Here, we demonstrate that NINJ1 also plays a crucial role in ferroptosis, but through a distinct mechanism. NINJ1 knockdown significantly protected cancer cells against ferroptosis induced by xCT inhibitors but no other classes of ferroptosis-inducing compounds (FINs). Glycine, known to inhibit canonical NINJ1-mediated membrane rupture in other cell deaths, had no impact on ferroptosis. A compound screen revealed that NINJ1-mediated ferroptosis protection can be abolished by pantothenate kinase inhibitor (PANKi), buthionine sulfoximine (BSO), and die","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Feb","modification":"2025-05-18T12:54:13.757Z","creation":"2025-05-18T12:54:13.757Z"},"accession":"S-EPMC10925083","cross_references":{"pubmed":["38464226"],"doi":["10.1101/2024.02.22.581432"]}}