{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Glausier JR"],"funding":["NIEHS NIH HHS","NIMH NIH HHS","NIAAA NIH HHS"],"pubmed_abstract":["Synaptic function is directly reflected in quantifiable ultrastructural features using electron microscopy (EM) approaches. This coupling of synaptic function and ultrastructure suggests that <i>in vivo</i> synaptic function can be inferred from EM analysis of <i>ex vivo</i> human brain tissue. To investigate this, we employed focused ion beam-scanning electron microscopy (FIB-SEM), a volume EM (VEM) approach, to generate ultrafine-resolution, three-dimensional (3D) micrographic datasets of postmortem human dorsolateral prefrontal cortex (DLPFC), a region with cytoarchitectonic characteristics distinct to human brain. Synaptic, sub-synaptic, and organelle measures were highly consistent with findings from experimental models that are free from antemortem or postmortem effects. Further, 3D neuropil reconstruction revealed a unique, ultrastructurally-complex, spiny dendritic shaft that exhibited features characteristic of heightened synaptic communication, integration, and plasticity. Altogether, our findings provide critical proof-of-concept data demonstrating that <i>ex vivo</i> VEM analysis is an effective approach to infer <i>in vivo</i> synaptic functioning in human brain."],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.02.26.582174"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10925168"],"repository":["biostudies-literature"],"pubmed_title":["Volume electron microscopy reveals 3D synaptic nanoarchitecture in postmortem human prefrontal cortex."],"pmcid":["PMC10925168"],"funding_grant_id":["R21 AA028800","R01 ES034037","S10 MH133575","R01 MH132544"],"pubmed_authors":["Banks-Tibbs T","Wu K","Glausier JR","Maier M","Freyberg Z","Melchitzky D","Bouchet-Marquis C","Ning J","Lewis DA"],"additional_accession":[]},"is_claimable":false,"name":"Volume electron microscopy reveals 3D synaptic nanoarchitecture in postmortem human prefrontal cortex.","description":"Synaptic function is directly reflected in quantifiable ultrastructural features using electron microscopy (EM) approaches. This coupling of synaptic function and ultrastructure suggests that <i>in vivo</i> synaptic function can be inferred from EM analysis of <i>ex vivo</i> human brain tissue. To investigate this, we employed focused ion beam-scanning electron microscopy (FIB-SEM), a volume EM (VEM) approach, to generate ultrafine-resolution, three-dimensional (3D) micrographic datasets of postmortem human dorsolateral prefrontal cortex (DLPFC), a region with cytoarchitectonic characteristics distinct to human brain. Synaptic, sub-synaptic, and organelle measures were highly consistent with findings from experimental models that are free from antemortem or postmortem effects. Further, 3D neuropil reconstruction revealed a unique, ultrastructurally-complex, spiny dendritic shaft that exhibited features characteristic of heightened synaptic communication, integration, and plasticity. Altogether, our findings provide critical proof-of-concept data demonstrating that <i>ex vivo</i> VEM analysis is an effective approach to infer <i>in vivo</i> synaptic functioning in human brain.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-04-08T19:54:38.342Z","creation":"2026-04-08T14:34:24.247Z"},"accession":"S-EPMC10925168","cross_references":{"pubmed":["38463986"],"doi":["10.1101/2024.02.26.582174"]}}