{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Bowman RL"],"funding":["NIGMS NIH HHS","NIH HHS"],"pubmed_abstract":["Specialized cellular protrusions facilitate local intercellular communications in various species, including mammals. Among these, airinemes play a crucial role in pigment pattern formation in zebrafish by mediating long-distance Notch signaling between pigment cells. Remarkably, airinemes exhibit large vesicle-like structure at their tips, which are pulled by a macrophage subpopulation and delivered to target cells. The interaction between macrophages and Delta-ligand carrying airineme vesicles is essential for initiating airineme-mediated signaling, yet the molecular detail of this interaction remains elusive. Through high-resolution live imaging and genetic <i>in vivo</i> manipulations, we found that adhesive interactions via the extracellular domain of CD44, a class I transmembrane glycoprotein, between macrophages and airineme vesicles are critical for airineme signaling. Mutants lacking the extracellular domain of CD44 lose their adhesiveness, resulting in a significant reduction in airineme extension and pigment pattern defects. Our findings provide valuable insights into the role of adhesive interactions between signal-sending cells and macrophages in long-range intercellular signaling."],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.02.27.582398"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10925269"],"repository":["biostudies-literature"],"pubmed_title":["CD44 facilitates adhesive interactions in airineme-mediated intercellular signaling."],"pmcid":["PMC10925269"],"funding_grant_id":["P40 OD010949","R35 GM142791"],"pubmed_authors":["Eom DS","Kim J","Bowman RL"],"additional_accession":[]},"is_claimable":false,"name":"CD44 facilitates adhesive interactions in airineme-mediated intercellular signaling.","description":"Specialized cellular protrusions facilitate local intercellular communications in various species, including mammals. Among these, airinemes play a crucial role in pigment pattern formation in zebrafish by mediating long-distance Notch signaling between pigment cells. Remarkably, airinemes exhibit large vesicle-like structure at their tips, which are pulled by a macrophage subpopulation and delivered to target cells. The interaction between macrophages and Delta-ligand carrying airineme vesicles is essential for initiating airineme-mediated signaling, yet the molecular detail of this interaction remains elusive. Through high-resolution live imaging and genetic <i>in vivo</i> manipulations, we found that adhesive interactions via the extracellular domain of CD44, a class I transmembrane glycoprotein, between macrophages and airineme vesicles are critical for airineme signaling. Mutants lacking the extracellular domain of CD44 lose their adhesiveness, resulting in a significant reduction in airineme extension and pigment pattern defects. Our findings provide valuable insights into the role of adhesive interactions between signal-sending cells and macrophages in long-range intercellular signaling.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2024-11-21T07:23:17.656Z","creation":"2024-11-21T07:23:17.656Z"},"accession":"S-EPMC10925269","cross_references":{"pubmed":["38463999"],"doi":["10.1101/2024.02.27.582398"]}}