{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shakhova ES"],"funding":["Promega","Planta","Light Bio","Russian Science Foundation (RSF)","Medical Research Council","Russian Science Foundation"],"pagination":["406-410"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10927554"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(3)"],"pubmed_abstract":["The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging."],"journal":["Nature methods"],"pubmed_title":["An improved pathway for autonomous bioluminescence imaging in eukaryotes."],"pmcid":["PMC10927554"],"funding_grant_id":["22-44-02024","22-14-00400","UKRI MC-A658-5QEA0"],"pubmed_authors":["Babenko VV","Hoang TT","Fakhranurova LI","Yampolsky IV","Malyshevskaia AK","Choob VV","Mitiouchkina T","Gao F","Karataeva TA","Gorbachev DA","Mishin AS","Bugaeva EN","Markina NM","Hall MP","Alekberova AE","Encell LP","Balakireva AV","Perfilov MM","Gorokhovatsky AY","Palkina KA","Sarkisyan KS","Wood KV","Boldyreva DI","Shakhova ES","Pletneva LK","Wood MG"],"additional_accession":[]},"is_claimable":false,"name":"An improved pathway for autonomous bioluminescence imaging in eukaryotes.","description":"The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-07-03T03:17:59.258Z","creation":"2025-04-04T12:58:54.757Z"},"accession":"S-EPMC10927554","cross_references":{"pubmed":["38253843"],"doi":["10.1038/s41592-023-02152-y"]}}