biostudies-literatureChen XResearch Project of Nantong Health Commissionthe Nantong Key Young Medical Talent ProgramWorkstation of Academician Liu YixunBasic Science Research Program of Nantong Citythe Fund of Xuzhou Science and TechnologyJiangsu province senile health scientific research projectScience and Technology Project of Nantong CityNational Natural Science Foundation of ChinaXuzhou Pengcheng Talents - Medical Young Reserve Talentsthe Open Fund of Jiangsu Key Laboratory for New Drug Research and Clinical PharmacyNatural Science Foundation of Jiangsu Provincee14057https://www.ebi.ac.uk/biostudies/studies/S-EPMC10928582biostudies-literatureUnknown23(3)Aging is a complex biological process leading to impaired functions, with a variety of hallmarks. In the testis of Drosophila, the terminal epithelium region is involved in spermatid release and maturation, while its functional diversity and regulatory mechanism remain poorly understood. In this study, we performed single-cell RNA-sequencing analysis (scRNA-seq) to characterize the transcriptomes of terminal epithelium in Drosophila testes at 2-, 10 and 40-Days. Terminal epithelium populations were defined with Metallothionein A (MtnA) and subdivided into six novel sub-cell clusters (EP0-EP5), and a series of marker genes were identified based on their expressions. The data revealed the functional characteristics of terminal epithelium populations, such as tight junction, focal adhesion, bAging cellSingle-cell transcriptome characteristics of testicular terminal epithelium lineages during aging in the Drosophila.PMC10928582XWRCSL20220162QA2021016BK20221376JC12022006LR20210352-2018.11.182101891KC20092JC202111982071838Zheng YSun CJi LZheng SGu ZHuang QYu JCheng XShi YLi ZQi YCao YChen XfalseSingle-cell transcriptome characteristics of testicular terminal epithelium lineages during aging in the Drosophila.Aging is a complex biological process leading to impaired functions, with a variety of hallmarks. In the testis of Drosophila, the terminal epithelium region is involved in spermatid release and maturation, while its functional diversity and regulatory mechanism remain poorly understood. In this study, we performed single-cell RNA-sequencing analysis (scRNA-seq) to characterize the transcriptomes of terminal epithelium in Drosophila testes at 2-, 10 and 40-Days. Terminal epithelium populations were defined with Metallothionein A (MtnA) and subdivided into six novel sub-cell clusters (EP0-EP5), and a series of marker genes were identified based on their expressions. The data revealed the functional characteristics of terminal epithelium populations, such as tight junction, focal adhesion, b2024-01-01T00:00:00Z2024 Mar2026-07-14T22:09:58.35Z2025-04-04T21:30:55.737ZS-EPMC109285823804457310.1111/acel.14057