biostudies-literatureUnknown27(4)Zhang YAsthenozoospermia, a male fertility disorder, has a complex and multifactorial etiology. Moreover, the effectiveness of different treatments for asthenozoospermia remains uncertain. Hence, by using bioinformatics techniques, the present study aimed to determine the underlying genetic markers and pathogenetic mechanisms associated with asthenozoospermia due to abnormal spermatogenesis and inflammation of the reproductive tract. GSE160749 dataset was downloaded from the Gene Expression Omnibus database, and the data were filtered to obtain 1336 differentially expressed genes (DEGs) associated with asthenozoospermia. These DEGs were intersected with the epithelial mesenchymal transition datasets to yield 61 candidate DEGs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enriExperimental and therapeutic medicine146https://www.ebi.ac.uk/biostudies/studies/S-EPMC10928817biostudies-literatureNovel underlying genetic markers for asthenozoospermia due to abnormal spermatogenesis and reproductive organ inflammation.PMC10928817Peng YZhang YWang YYan HXu JfalseNovel underlying genetic markers for asthenozoospermia due to abnormal spermatogenesis and reproductive organ inflammation.Asthenozoospermia, a male fertility disorder, has a complex and multifactorial etiology. Moreover, the effectiveness of different treatments for asthenozoospermia remains uncertain. Hence, by using bioinformatics techniques, the present study aimed to determine the underlying genetic markers and pathogenetic mechanisms associated with asthenozoospermia due to abnormal spermatogenesis and inflammation of the reproductive tract. GSE160749 dataset was downloaded from the Gene Expression Omnibus database, and the data were filtered to obtain 1336 differentially expressed genes (DEGs) associated with asthenozoospermia. These DEGs were intersected with the epithelial mesenchymal transition datasets to yield 61 candidate DEGs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enri2024-01-01T00:00:00Z2024 Apr2026-07-15T04:19:43.929Z2024-11-21T07:23:02.978ZS-EPMC109288173847692310.3892/etm.2024.12434