{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Treisman M"],"funding":["Australian Government","Australian Research Council"],"pagination":["1828-1833"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10929621"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(9)"],"pubmed_abstract":["Cytochrome-P450-mediated cross-linking of ribosomally encoded peptides (RiPPs) is rapidly expanding and displays great potential for biocatalysis. Here, we demonstrate that active site engineering of the biarylitide cross-linking enzyme P450<sub>Blt</sub> enables the formation of His-X-Tyr and Tyr-X-Tyr cross-linked peptides, thus showing how such P450s can be further exploited to produce alternate cyclic tripeptides with controlled cross-linking states."],"journal":["Organic letters"],"pubmed_title":["An Engineered Biarylitide Cross-Linking P450 from RiPP Biosynthesis Generates Alternative Cyclic Peptides."],"pmcid":["PMC10929621"],"funding_grant_id":["CE200100012"],"pubmed_authors":["Coe L","Hess C","Machell DL","Sasi VM","Hansen MH","Ly A","Gullick J","Leichthammer V","Zhao Y","Hooper J","Treisman M","Cryle MJ","Schittenhelm RB","Jackson CJ","De Voss JJ","Tailhades J"],"additional_accession":[]},"is_claimable":false,"name":"An Engineered Biarylitide Cross-Linking P450 from RiPP Biosynthesis Generates Alternative Cyclic Peptides.","description":"Cytochrome-P450-mediated cross-linking of ribosomally encoded peptides (RiPPs) is rapidly expanding and displays great potential for biocatalysis. Here, we demonstrate that active site engineering of the biarylitide cross-linking enzyme P450<sub>Blt</sub> enables the formation of His-X-Tyr and Tyr-X-Tyr cross-linked peptides, thus showing how such P450s can be further exploited to produce alternate cyclic tripeptides with controlled cross-linking states.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-22T20:09:10.123Z","creation":"2025-04-06T03:01:49.567Z"},"accession":"S-EPMC10929621","cross_references":{"pubmed":["38417822"],"doi":["10.1021/acs.orglett.3c04366"]}}