biostudies-literatureZhang XHainan Province Science and Technology Special FundChinese Academy of Tropical Agricultural Sciences for Science and Technology Innovation Team of National Tropical Agricultural Science CenterCentral Public-interest Scientific Institution Basal Research FundSanya Yazhou Bay Science and Technology City701https://www.ebi.ac.uk/biostudies/studies/S-EPMC10930650biostudies-literatureUnknown13(5)This study investigated the immunoprotective effects of the extract of <i>Vanilla planifolia Andrew</i> (EVPA) on cyclophosphamide (Cy)-induced immunosuppression in mice. The results show that EVPA administration significantly alleviated the immune damage induced by Cy, as evidenced by an improved body weight, organ index, and colonic injury. A further analysis of microbial diversity revealed that the EVPA primarily increased the abundance of the beneficial bacteria <i>Verrucomicrobiota</i>, <i>Lactobacillaceae</i>, and <i>Lactobacillus</i> while decreasing <i>Akkermansiaceae</i>, <i>Akkermansia</i>, <i>Romboutsia</i>, and <i>Lactococcus</i>, thereby ameliorating the microbial dysbiosis caused by Cy. A metabolomic analysis revealed significant alterations in the microbial metabolite levelsFoods (Basel, Switzerland)Microbiome-Metabolomic Analysis Revealed the Immunoprotective Effects of the Extract of <i>Vanilla planifolia Andrew</i> (EVPA) on Immunosuppressed Mice.PMC10930650ZDYF2022XDNY268CATASCXTD2023041630142022008SCKJ-JYRC-2022-25Li YLi CZhao QGu FZhang XZhu KXu FChu ZfalseMicrobiome-Metabolomic Analysis Revealed the Immunoprotective Effects of the Extract of <i>Vanilla planifolia Andrew</i> (EVPA) on Immunosuppressed Mice.This study investigated the immunoprotective effects of the extract of <i>Vanilla planifolia Andrew</i> (EVPA) on cyclophosphamide (Cy)-induced immunosuppression in mice. The results show that EVPA administration significantly alleviated the immune damage induced by Cy, as evidenced by an improved body weight, organ index, and colonic injury. A further analysis of microbial diversity revealed that the EVPA primarily increased the abundance of the beneficial bacteria <i>Verrucomicrobiota</i>, <i>Lactobacillaceae</i>, and <i>Lactobacillus</i> while decreasing <i>Akkermansiaceae</i>, <i>Akkermansia</i>, <i>Romboutsia</i>, and <i>Lactococcus</i>, thereby ameliorating the microbial dysbiosis caused by Cy. A metabolomic analysis revealed significant alterations in the microbial metabolite levels2024-01-01T00:00:00Z2024 Feb2026-07-09T10:45:27.293Z2026-07-09T10:26:21.002ZS-EPMC109306503847281410.3390/foods13050701