biostudies-literatureUnknown14(5)Kehl AGenetic molecular testing is starting to gain traction as part of standard clinical practice for dogs with cancer due to its multi-faceted benefits, such as potentially being able to provide diagnostic, prognostic and/or therapeutic information. However, the benefits and ultimate success of genomic analysis in the clinical setting are reliant on the robustness of the tools used to generate the results, which continually expand as new technologies are developed. To this end, we review the different materials from which tumour cells, DNA, RNA and the relevant proteins can be isolated and what methods are available for interrogating their molecular profile, including analysis of the genetic alterations (both somatic and germline), transcriptional changes and epigenetic modifications (includinAnimals : an open access journal from MDPI769https://www.ebi.ac.uk/biostudies/studies/S-EPMC10930838biostudies-literatureReview of Molecular Technologies for Investigating Canine Cancer.PMC10930838Kehl Ade Brot SAupperle-Lellbach Hvan der Weyden LfalseReview of Molecular Technologies for Investigating Canine Cancer.Genetic molecular testing is starting to gain traction as part of standard clinical practice for dogs with cancer due to its multi-faceted benefits, such as potentially being able to provide diagnostic, prognostic and/or therapeutic information. However, the benefits and ultimate success of genomic analysis in the clinical setting are reliant on the robustness of the tools used to generate the results, which continually expand as new technologies are developed. To this end, we review the different materials from which tumour cells, DNA, RNA and the relevant proteins can be isolated and what methods are available for interrogating their molecular profile, including analysis of the genetic alterations (both somatic and germline), transcriptional changes and epigenetic modifications (includin2024-01-01T00:00:00Z2024 Feb2025-04-19T05:48:30.82Z2025-04-19T05:48:30.82ZS-EPMC109308383847315410.3390/ani14050769