biostudies-literatureUnknown61(5)Bilic Rajs BThe aim of this work was to give characteristic stable carbon and nitrogen isotope ratio (<i>δ</i>¹³C_honey, <i>δ</i>¹³C_protein and <i>δ</i>¹⁵N) ranges and examine their relation with botanical origin of honey. Despite that <i>δ</i>¹³C parameter has primary purpose to detect honey adulteration, stable isotopes generally have become important parameter for detection its botanical and geographical origin. The data about stable isotopes are scarce in comparison to other well-known parameters in honey, and in Croatia there is no data about stable isotopes in unifloral honey. This research includes six characteristic honey types (black locust, chestnut, lime, rape, winter savory, and sage honey) from Croatia. Large number of differences between honey types were found in the analyzed IRMS parameters. PCA analysis has successfully separated winter savory from all other honey types, except sage honey, whose samples differed from black locust samples.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s13197-023-05888-9.Journal of food science and technology939-949https://www.ebi.ac.uk/biostudies/studies/S-EPMC10933234biostudies-literatureAnalysis of δ&lt;sup&gt;13&lt;/sup&gt;C and δ&lt;sup&gt;15&lt;/sup&gt;N values in Croatian honey by EA-IRMS and possibility of their application in botanical origin verification.PMC10933234Flanjak IBilic Rajs BCacic-Kenjeric FMilojkovic-Opsenica DPrimorac LTesic ZfalseAnalysis of δ&lt;sup&gt;13&lt;/sup&gt;C and δ&lt;sup&gt;15&lt;/sup&gt;N values in Croatian honey by EA-IRMS and possibility of their application in botanical origin verification.The aim of this work was to give characteristic stable carbon and nitrogen isotope ratio (<i>δ</i>¹³C_honey, <i>δ</i>¹³C_protein and <i>δ</i>¹⁵N) ranges and examine their relation with botanical origin of honey. Despite that <i>δ</i>¹³C parameter has primary purpose to detect honey adulteration, stable isotopes generally have become important parameter for detection its botanical and geographical origin. The data about stable isotopes are scarce in comparison to other well-known parameters in honey, and in Croatia there is no data about stable isotopes in unifloral honey. This research includes six characteristic honey types (black locust, chestnut, lime, rape, winter savory, and sage honey) from Croatia. Large number of differences between honey types were found in the analyzed IRMS parameters. PCA analysis has successfully separated winter savory from all other honey types, except sage honey, whose samples differed from black locust samples.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s13197-023-05888-9.2024-01-01T00:00:00Z2024 May2025-06-27T03:05:33.378Z2025-06-27T03:05:33.378ZS-EPMC109332343848729110.1007/s13197-023-05888-9