biostudies-literatureBakir GNatural Sciences and Engineering Research Council of Canada355-364https://www.ebi.ac.uk/biostudies/studies/S-EPMC10935619biostudies-literatureUnknown78(4)The cell wall integrity (CWI) signaling pathway regulates yeast cell wall biosynthesis, cell division, and responses to external stress. The cell wall, comprised of a dense network of chitin, β-1,3- and β-1,6- glucans, and mannoproteins, is very thin, <100 nm. Alterations in cell wall composition may activate the CWI pathway. <i>Saccharomyces cerevisiae</i>, a model yeast, was used to study the role of individual wall components in altering the structure and biophysical properties of the yeast cell wall. Near-field Fourier transform infrared spectroscopy (nano-FT-IR) was used for the first direct, spectrochemical identification of cell wall composition in a background (wild-type) strain and two deletion mutants from the yeast knock-out collection: <i>kre6</i>Δ and <i>knr4</i>Δ. Killer toxiApplied spectroscopy<i>Saccharomyces cerevisiae</i> CellWall Remodeling in the Absence of Knr4 and Kre6 Revealed by Nano-FourierTransform Infrared Spectroscopy.PMC1093561906649-201805931-2017Martin-Yken HBechtel HABakir GGough KMDahms TESfalse<i>Saccharomyces cerevisiae</i> CellWall Remodeling in the Absence of Knr4 and Kre6 Revealed by Nano-FourierTransform Infrared Spectroscopy.The cell wall integrity (CWI) signaling pathway regulates yeast cell wall biosynthesis, cell division, and responses to external stress. The cell wall, comprised of a dense network of chitin, β-1,3- and β-1,6- glucans, and mannoproteins, is very thin, <100 nm. Alterations in cell wall composition may activate the CWI pathway. <i>Saccharomyces cerevisiae</i>, a model yeast, was used to study the role of individual wall components in altering the structure and biophysical properties of the yeast cell wall. Near-field Fourier transform infrared spectroscopy (nano-FT-IR) was used for the first direct, spectrochemical identification of cell wall composition in a background (wild-type) strain and two deletion mutants from the yeast knock-out collection: <i>kre6</i>Δ and <i>knr4</i>Δ. Killer toxi2024-01-01T00:00:00Z2024 Apr2026-07-15T00:42:27.013Z2026-06-27T03:05:46.529ZS-EPMC109356193837801410.1177/00037028231213658