biostudies-literatureUnknown24(1)Wang R<h4>Background</h4>Bladder cancer (BC) is one of the most common malignancies of the genitourinary system. Phosphofructokinase 1 (PFK-1) is one of member of PFK, which plays an important role in reprogramming cancer metabolism, such as lactylation modification. Zinc finger E-box-binding homeobox 1 (ZEB1) has been demonstrated to be a oncogene in many cancers. Therefore, this study was performed to explore the effects of PFK-1 on the lactylation of ZEB1 in BC development.<h4>Methods</h4>Cell viability was measured using the CCK-8 kit. The glucose assay kit and lactate assay kit were used to detect glucose utilization and lactate production. The DNA was purified and quantified by qRT-PCR.<h4>Results</h4>In the present study, we found that ZEB1 expression levels were significantly elevated inBMC urology59https://www.ebi.ac.uk/biostudies/studies/S-EPMC10935987biostudies-literatureThe mechanism of PFK-1 in the occurrence and development of bladder cancer by regulating ZEB1 lactylation.PMC10935987Yang ZWang RCao JXu FHu LShe YfalseThe mechanism of PFK-1 in the occurrence and development of bladder cancer by regulating ZEB1 lactylation.<h4>Background</h4>Bladder cancer (BC) is one of the most common malignancies of the genitourinary system. Phosphofructokinase 1 (PFK-1) is one of member of PFK, which plays an important role in reprogramming cancer metabolism, such as lactylation modification. Zinc finger E-box-binding homeobox 1 (ZEB1) has been demonstrated to be a oncogene in many cancers. Therefore, this study was performed to explore the effects of PFK-1 on the lactylation of ZEB1 in BC development.<h4>Methods</h4>Cell viability was measured using the CCK-8 kit. The glucose assay kit and lactate assay kit were used to detect glucose utilization and lactate production. The DNA was purified and quantified by qRT-PCR.<h4>Results</h4>In the present study, we found that ZEB1 expression levels were significantly elevated in2024-01-01T00:00:00Z2024 Mar2026-07-14T21:22:16.197Z2026-06-24T03:12:51.19ZS-EPMC109359873848118210.1186/s12894-024-01444-5