biostudies-literatureZhan QTesting for AmericaNCATS NIH HHSBert L and N Kuggie Vallee FoundationNIDA NIH HHSNIAID NIH HHSRockefeller FoundationPershing Square FoundationNational Institutes of HealthOpenCovidScreen FoundationUniversity of MiamiWorldQuant FoundationAlfred P. Sloan FoundationNational Institute on Drug Abuse2849-2862https://www.ebi.ac.uk/biostudies/studies/S-EPMC10936583biostudies-literatureUnknown3(9)Wastewater-based epidemiology (WBE) has been utilized to track community infections of Coronavirus Disease 2019 (COVID-19) by detecting RNA of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), within samples collected from wastewater. The correlations between community infections and wastewater measurements of the RNA can potentially change as SARS-CoV-2 evolves into new variations by mutating. This study analyzed SARS-CoV-2 RNA, and indicators of human waste in wastewater from two sewersheds of different scales (University of Miami (UM) campus and Miami-Dade County Central District wastewater treatment plant (CDWWTP)) during five internally defined COVID-19 variant dominant periods (Initial, Pre-Delta, Delta, Omicron and Post-Omicron wave). SARS-CoV-2 RNA quantities were cACS ES&T waterCorrelative analysis of wastewater trends with clinical cases and hospitalizations through five dominant variant waves of COVID-19.PMC10936583R01AI151059R01 AI125416U01 DA053941U01DA053941501c3R21 AI129851R01AI125416R21AI129851R01 AI151059G-2015-13964UM1 TR004556Boone MMCurrall BBLamar WEBabler KMThomas CKumar NZarnegarnia YBeaver CCSharkey MEStevenson MLaine JRoca MAGrills GSWilliams SLZhan QSolle NSYin XCosculluela GAMason CEShukla BSAmirali ACortizas EMReding BDSchurer SCComerford SSolo-Gabriele HMLyu JTallon JJVidovic DCooper DSuarez MMKobetz EfalseCorrelative analysis of wastewater trends with clinical cases and hospitalizations through five dominant variant waves of COVID-19.Wastewater-based epidemiology (WBE) has been utilized to track community infections of Coronavirus Disease 2019 (COVID-19) by detecting RNA of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), within samples collected from wastewater. The correlations between community infections and wastewater measurements of the RNA can potentially change as SARS-CoV-2 evolves into new variations by mutating. This study analyzed SARS-CoV-2 RNA, and indicators of human waste in wastewater from two sewersheds of different scales (University of Miami (UM) campus and Miami-Dade County Central District wastewater treatment plant (CDWWTP)) during five internally defined COVID-19 variant dominant periods (Initial, Pre-Delta, Delta, Omicron and Post-Omicron wave). SARS-CoV-2 RNA quantities were c2023-01-01T00:00:00Z2023 Sep2025-04-04T01:35:45.9Z2025-02-19T02:34:20.126ZS-EPMC109365833848769610.1021/acsestwater.3c00032