{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wong KG"],"funding":["NIA NIH HHS","NHLBI NIH HHS"],"pagination":["eadm9518"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10936876"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(11)"],"pubmed_abstract":["Extracellular signals are transmitted through kinase cascades to modulate gene expression, but it remains unclear how epigenetic changes regulate this response. Here, we provide evidence that growth factor-stimulated changes in the transcript levels of many responsive genes are accompanied by increases in histone phosphorylation levels, specifically at histone H3 serine-10 when the adjacent lysine-9 is dimethylated (H3K9me2S10). Imaging and proteomic approaches show that epidermal growth factor (EGF) stimulation results in H3K9me2S10 phosphorylation, which occurs in genomic regions enriched for regulatory enhancers of EGF-responsive genes. We also demonstrate that the EGF-induced increase in H3K9me2S10ph is dependent on the nuclear kinase MSK2, and this subset of EGF-induced genes is dependent on MSK2 for transcription. Together, our work indicates that growth factor-induced changes in chromatin state can mediate the activation of downstream genes."],"journal":["Science advances"],"pubmed_title":["Growth factor-induced activation of MSK2 leads to phosphorylation of H3K9me2S10 and corresponding changes in gene expression."],"pmcid":["PMC10936876"],"funding_grant_id":["R21 AG081795","R35 HL140018"],"pubmed_authors":["Epstein JA","Li J","Kiseleva AA","Cheng YF","Wu VH","Wong KG","Poleshko A","Smith CL"],"additional_accession":[]},"is_claimable":false,"name":"Growth factor-induced activation of MSK2 leads to phosphorylation of H3K9me2S10 and corresponding changes in gene expression.","description":"Extracellular signals are transmitted through kinase cascades to modulate gene expression, but it remains unclear how epigenetic changes regulate this response. Here, we provide evidence that growth factor-stimulated changes in the transcript levels of many responsive genes are accompanied by increases in histone phosphorylation levels, specifically at histone H3 serine-10 when the adjacent lysine-9 is dimethylated (H3K9me2S10). Imaging and proteomic approaches show that epidermal growth factor (EGF) stimulation results in H3K9me2S10 phosphorylation, which occurs in genomic regions enriched for regulatory enhancers of EGF-responsive genes. We also demonstrate that the EGF-induced increase in H3K9me2S10ph is dependent on the nuclear kinase MSK2, and this subset of EGF-induced genes is dependent on MSK2 for transcription. Together, our work indicates that growth factor-induced changes in chromatin state can mediate the activation of downstream genes.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2024-11-14T20:31:39.164Z","creation":"2024-11-14T20:31:39.164Z"},"accession":"S-EPMC10936876","cross_references":{"pubmed":["38478612"],"doi":["10.1126/sciadv.adm9518"]}}