{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(11)"],"submitter":["Sugiyama S"],"pubmed_abstract":["Endoplasmic reticulum (ER)-plasma membrane (PM) tethering is crucial for the non-vesicular lipid transport between the ER membrane and the PM. However, the PM-associated ER can impede the PM binding of cytoskeletons and other organelles. It is poorly understood how the competition between the ER and cytoskeletons/organelles on the PM is resolved. Here, we show that, upon septin collar assembly, ER-PM tethering proteins are excluded from the yeast bud sites, and the PM-associated ER is locally detached from the PM. Our results suggest that PM flows by polarized exocytosis extrude PM proteins, including ER-PM tethering proteins, from the bud sites. When the reorganization of the ER-PM tethering was inhibited by exocytosis repression, septin localization was restricted to the PM sites poor in"],"journal":["Science advances"],"pagination":["eadj1512"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10936878"],"repository":["biostudies-literature"],"pubmed_title":["Exocytic plasma membrane flows remodel endoplasmic reticulum-plasma membrane tethering for septin collar assembly."],"pmcid":["PMC10936878"],"pubmed_authors":["Sugiyama S","Kono K"],"additional_accession":[]},"is_claimable":false,"name":"Exocytic plasma membrane flows remodel endoplasmic reticulum-plasma membrane tethering for septin collar assembly.","description":"Endoplasmic reticulum (ER)-plasma membrane (PM) tethering is crucial for the non-vesicular lipid transport between the ER membrane and the PM. However, the PM-associated ER can impede the PM binding of cytoskeletons and other organelles. It is poorly understood how the competition between the ER and cytoskeletons/organelles on the PM is resolved. Here, we show that, upon septin collar assembly, ER-PM tethering proteins are excluded from the yeast bud sites, and the PM-associated ER is locally detached from the PM. Our results suggest that PM flows by polarized exocytosis extrude PM proteins, including ER-PM tethering proteins, from the bud sites. When the reorganization of the ER-PM tethering was inhibited by exocytosis repression, septin localization was restricted to the PM sites poor in","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-05T12:06:31.549Z","creation":"2025-04-05T12:06:31.549Z"},"accession":"S-EPMC10936878","cross_references":{"pubmed":["38478607"],"doi":["10.1126/sciadv.adj1512"]}}