{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yamazaki S"],"funding":["Core Research for Evolutional Science and Technology","Takeda Science Foundation","Japan Agency for Medical Research and Development","Uehara Memorial Foundation","Japan Science and Technology Agency"],"pagination":["414-425"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10937152"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(3)"],"pubmed_abstract":["Myeloid cells, which originate from hematopoietic stem/progenitor cells (HSPCs), play a crucial role in mitigating infections. This study aimed to explore the impact of mesenchymal stem/stromal cells (MSCs) on the differentiation of HSPCs and progenitors through the C-C motif chemokine CCL2/CCR2 signaling pathway. Murine MSCs, identified as PDGFRα<sup>+</sup>Sca-1<sup>+</sup> cells (PαS cells), were found to secrete CCL2, particularly in response to lipopolysaccharide stimulation. MSC-secreted CCL2 promoted the differentiation of granulocyte/macrophage progenitors into the myeloid lineage. MSC-derived CCL2 plays an important role in the early phase of myeloid cell differentiation in vivo. Single-cell RNA sequencing analysis confirmed that CCL2-mediated cell fate determination was also observed in human bone marrow cells. These findings provide valuable insights for investigating the in vivo effects of MSC transplantation."],"journal":["Stem cell reports"],"pubmed_title":["Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling."],"pmcid":["PMC10937152"],"funding_grant_id":["JP20bm0404031h0103","JP18KK0449","JP21H03328","JP18bm0404022h0001","23bm1223011h0001","JPMJCR2124","JP19KK0216","JP19K10024"],"pubmed_authors":["Azuma Y","Mabuchi Y","Morishita S","Kikuchi R","Suto EG","Kimura T","Kondo A","Hisamatsu D","Akazawa C","Komatsu N","Nishikii H","Naraoka Y","Araki M","Yamazaki S"],"additional_accession":[]},"is_claimable":false,"name":"Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling.","description":"Myeloid cells, which originate from hematopoietic stem/progenitor cells (HSPCs), play a crucial role in mitigating infections. This study aimed to explore the impact of mesenchymal stem/stromal cells (MSCs) on the differentiation of HSPCs and progenitors through the C-C motif chemokine CCL2/CCR2 signaling pathway. Murine MSCs, identified as PDGFRα<sup>+</sup>Sca-1<sup>+</sup> cells (PαS cells), were found to secrete CCL2, particularly in response to lipopolysaccharide stimulation. MSC-secreted CCL2 promoted the differentiation of granulocyte/macrophage progenitors into the myeloid lineage. MSC-derived CCL2 plays an important role in the early phase of myeloid cell differentiation in vivo. Single-cell RNA sequencing analysis confirmed that CCL2-mediated cell fate determination was also observed in human bone marrow cells. These findings provide valuable insights for investigating the in vivo effects of MSC transplantation.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-24T03:08:30.56Z","creation":"2026-06-24T03:06:11.122Z"},"accession":"S-EPMC10937152","cross_references":{"pubmed":["38428413"],"doi":["10.1016/j.stemcr.2024.02.002"]}}