{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Goosen WJ"],"funding":["European & Developing Countries Clinical Trials Partnership (EDCTP)","South African Medical Research Council","European Commission","National Research Foundation","Wellcome Trust"],"pagination":["100702"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10937233"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18"],"pubmed_abstract":["This study investigated the presence of <i>Mycobacterium bovis</i> (<i>M. bovis</i>) DNA in archived human sputum samples previously collected from residents who reside adjacent to the <i>M. bovis</i>-endemic Hluhluwe-iMfolozi wildlife park, South Africa (SA). Sixty-eight sputum samples were GeneXpert MTB/RIF Ultra-positive for <i>M. tuberculosis</i> complex (MTBC) DNA but culture negative for <i>M. tuberculosis</i>. Amplification and Sanger sequencing of <i>hsp65</i> and <i>rpoB</i> genes from DNA extracted from stored heat-inactivated sputum samples confirmed the presence of detectable amounts of MTBC from 20 out of the 68 sputum samples. Region of difference PCR, spoligotyping and <i>gyrB</i> long-read amplicon deep sequencing identified <i>M. bovis</i> (<i>n</i> = 10) and <i>M. tubercu"],"journal":["One health (Amsterdam, Netherlands)"],"pubmed_title":["Identification and molecular characterization of <i>Mycobacterium bovis</i> DNA in GeneXpert® MTB/RIF ultra-positive, culture-negative sputum from a rural community in South Africa."],"pmcid":["PMC10937233"],"funding_grant_id":["1022002","201433/Z/16/A","101103171","86949","222941/Z/21/Z"],"pubmed_authors":["Hanekom WA","Vukuzazi Team","Wong EB","Smit T","Miller MA","Ghielmetti G","Streicher EM","Warren RM","Moodley S","Goosen WJ","Moosa Y","Zulu T","Kleynhans L","Kerr TJ"],"additional_accession":[]},"is_claimable":false,"name":"Identification and molecular characterization of <i>Mycobacterium bovis</i> DNA in GeneXpert® MTB/RIF ultra-positive, culture-negative sputum from a rural community in South Africa.","description":"This study investigated the presence of <i>Mycobacterium bovis</i> (<i>M. bovis</i>) DNA in archived human sputum samples previously collected from residents who reside adjacent to the <i>M. bovis</i>-endemic Hluhluwe-iMfolozi wildlife park, South Africa (SA). Sixty-eight sputum samples were GeneXpert MTB/RIF Ultra-positive for <i>M. tuberculosis</i> complex (MTBC) DNA but culture negative for <i>M. tuberculosis</i>. Amplification and Sanger sequencing of <i>hsp65</i> and <i>rpoB</i> genes from DNA extracted from stored heat-inactivated sputum samples confirmed the presence of detectable amounts of MTBC from 20 out of the 68 sputum samples. Region of difference PCR, spoligotyping and <i>gyrB</i> long-read amplicon deep sequencing identified <i>M. bovis</i> (<i>n</i> = 10) and <i>M. tubercu","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-07-15T00:44:56.089Z","creation":"2026-06-27T03:06:16.885Z"},"accession":"S-EPMC10937233","cross_references":{"pubmed":["38487729"],"doi":["10.1016/j.onehlt.2024.100702"]}}