biostudies-literatureInman JLIntelligence Advanced Research Projects ActivityNIGMS NIH HHS6119https://www.ebi.ac.uk/biostudies/studies/S-EPMC10937999biostudies-literatureUnknown14(1)Non-invasive methods of detecting radiation exposure show promise to improve upon current approaches to biological dosimetry in ease, speed, and accuracy. Here we developed a pipeline that employs Fourier transform infrared (FTIR) spectroscopy in the mid-infrared spectrum to identify a signature of low dose ionizing radiation exposure in mouse ear pinnae over time. Mice exposed to 0.1 to 2 Gy total body irradiation were repeatedly measured by FTIR at the stratum corneum of the ear pinnae. We found significant discriminative power for all doses and time-points out to 90 days after exposure. Classification accuracy was maximized when testing 14 days after exposure (specificity > 0.9 with a sensitivity threshold of 0.9) and dropped by roughly 30% sensitivity at 90 days. Infrared frequencies pScientific reportsLong-term, non-invasive FTIR detection of low-dose ionizing radiation exposure.PMC1093799920008-D2021-2107310008R01 GM126218Inman JLGhosh DCelniker SEZwart PHNakamura KWan KHDe Chant JChen LSnijders AMMao JHObst-Huebl LRalston CYBrydon EHolman HNWu YChang HBrown JBfalseLong-term, non-invasive FTIR detection of low-dose ionizing radiation exposure.Non-invasive methods of detecting radiation exposure show promise to improve upon current approaches to biological dosimetry in ease, speed, and accuracy. Here we developed a pipeline that employs Fourier transform infrared (FTIR) spectroscopy in the mid-infrared spectrum to identify a signature of low dose ionizing radiation exposure in mouse ear pinnae over time. Mice exposed to 0.1 to 2 Gy total body irradiation were repeatedly measured by FTIR at the stratum corneum of the ear pinnae. We found significant discriminative power for all doses and time-points out to 90 days after exposure. Classification accuracy was maximized when testing 14 days after exposure (specificity > 0.9 with a sensitivity threshold of 0.9) and dropped by roughly 30% sensitivity at 90 days. Infrared frequencies p2024-01-01T00:00:00Z2024 Mar2026-07-14T20:57:15.529Z2026-06-24T03:06:59.985ZS-EPMC109379993848082710.1038/s41598-024-56491-7