{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kim JW"],"funding":["National Institute of Diabetes and Digestive and Kidney Diseases","NIDDK NIH HHS","NHLBI NIH HHS","National Institutes of Health"],"pagination":["104147"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10939783"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["131"],"pubmed_abstract":["Hematopoietic stem cell transplantation requires the collection of hematopoietic cells from patients or stem cell donors. Granulocyte colony-stimulating factor (G-CSF) is widely used in the clinic to mobilize hematopoietic stem and progenitor cells (HSPCs) from the adult bone marrow niche into circulation, allowing a collection of HSPCs from the blood. The mechanism by which G-CSF acts to mobilize HSPCs is unclear, with some studies showing a direct stimulation of stem cells and others suggesting that myeloid cells are required. In this study, we developed a heat-inducible G-CSF transgenic zebrafish line to study HSPC mobilization in vivo. Live imaging of HSPCs after G-CSF induction revealed an increase in circulating HSPCs, demonstrating a successful HSPC mobilization. These mobilized HSPCs went on to prematurely colonize the kidney marrow, the adult zebrafish hematopoietic niche. We eliminated neutrophils or macrophages using a nitroreductase-based cell ablation system and found that G-CSF still mobilizes HSPCs from the niche. Our findings indicate that neutrophils and macrophages are not required for G-CSF-induced HSPC mobilization from the embryonic hematopoietic niche."],"journal":["Experimental hematology"],"pubmed_title":["G-CSF-induced hematopoietic stem cell mobilization from the embryonic hematopoietic niche does not require neutrophils and macrophages."],"pmcid":["PMC10939783"],"funding_grant_id":["RC2 DK120535","R01 HL048801","R01 HL144780","P01 HL131477","RC2DK120535"],"pubmed_authors":["Fedorov EA","Zon LI","Kim JW"],"additional_accession":[]},"is_claimable":false,"name":"G-CSF-induced hematopoietic stem cell mobilization from the embryonic hematopoietic niche does not require neutrophils and macrophages.","description":"Hematopoietic stem cell transplantation requires the collection of hematopoietic cells from patients or stem cell donors. Granulocyte colony-stimulating factor (G-CSF) is widely used in the clinic to mobilize hematopoietic stem and progenitor cells (HSPCs) from the adult bone marrow niche into circulation, allowing a collection of HSPCs from the blood. The mechanism by which G-CSF acts to mobilize HSPCs is unclear, with some studies showing a direct stimulation of stem cells and others suggesting that myeloid cells are required. In this study, we developed a heat-inducible G-CSF transgenic zebrafish line to study HSPC mobilization in vivo. Live imaging of HSPCs after G-CSF induction revealed an increase in circulating HSPCs, demonstrating a successful HSPC mobilization. These mobilized HSPCs went on to prematurely colonize the kidney marrow, the adult zebrafish hematopoietic niche. We eliminated neutrophils or macrophages using a nitroreductase-based cell ablation system and found that G-CSF still mobilizes HSPCs from the niche. Our findings indicate that neutrophils and macrophages are not required for G-CSF-induced HSPC mobilization from the embryonic hematopoietic niche.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-04T01:12:37.579Z","creation":"2025-04-04T01:12:37.579Z"},"accession":"S-EPMC10939783","cross_references":{"pubmed":["38160994"],"doi":["10.1016/j.exphem.2023.104147"]}}