{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["McNally DL"],"funding":["NIDCR NIH HHS","Foundation for the National Institutes of Health","Defense Sciences Office, DARPA"],"pagination":["e2302528"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10939856"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(7)"],"pubmed_abstract":["In nature, some organisms survive extreme environments by inducing a biostatic state wherein cellular contents are effectively vitrified. Recently, a synthetic biostatic state in mammalian cells is achieved via intracellular network formation using bio-orthogonal strain-promoted azide-alkyne cycloaddition (SPAAC) reactions between functionalized poly(ethylene glycol) (PEG) macromers. In this work, the effects of intracellular network formation on a 3D epithelial MCF10A spheroid model are explored. Macromer-transfected cells are encapsulated in Matrigel, and spheroid area is reduced by ≈50% compared to controls. The intracellular hydrogel network increases the quiescent cell population, as indicated by increased p21 expression. Additionally, bioenergetics (ATP/ADP ratio) and functional metabolic rates are reduced. To enable reversibility of the biostasis effect, a photosensitive nitrobenzyl-containing macromer is incorporated into the PEG network, allowing for light-induced degradation. Following light exposure, cell state, and proliferation return to control levels, while SPAAC-treated spheroids without light exposure (i.e., containing intact intracellular networks) remain smaller and less proliferative through this same period. These results demonstrate that photodegradable intracellular hydrogels can induce a reversible slow-growing state in 3D spheroid culture."],"journal":["Advanced healthcare materials"],"pubmed_title":["Reversible Intracellular Gelation of MCF10A Cells Enables Programmable Control Over 3D Spheroid Growth."],"pmcid":["PMC10939856"],"funding_grant_id":["R01 DE016523","W911NF‐19‐2‐0024"],"pubmed_authors":["Kirkpatrick BE","McNally DL","Macdougall LJ","Maduka CV","Spencer SL","Anseth KS","Bowman CN","Fairbanks BD","Hoffman TE"],"additional_accession":[]},"is_claimable":false,"name":"Reversible Intracellular Gelation of MCF10A Cells Enables Programmable Control Over 3D Spheroid Growth.","description":"In nature, some organisms survive extreme environments by inducing a biostatic state wherein cellular contents are effectively vitrified. Recently, a synthetic biostatic state in mammalian cells is achieved via intracellular network formation using bio-orthogonal strain-promoted azide-alkyne cycloaddition (SPAAC) reactions between functionalized poly(ethylene glycol) (PEG) macromers. In this work, the effects of intracellular network formation on a 3D epithelial MCF10A spheroid model are explored. Macromer-transfected cells are encapsulated in Matrigel, and spheroid area is reduced by ≈50% compared to controls. The intracellular hydrogel network increases the quiescent cell population, as indicated by increased p21 expression. Additionally, bioenergetics (ATP/ADP ratio) and functional metabolic rates are reduced. To enable reversibility of the biostasis effect, a photosensitive nitrobenzyl-containing macromer is incorporated into the PEG network, allowing for light-induced degradation. Following light exposure, cell state, and proliferation return to control levels, while SPAAC-treated spheroids without light exposure (i.e., containing intact intracellular networks) remain smaller and less proliferative through this same period. These results demonstrate that photodegradable intracellular hydrogels can induce a reversible slow-growing state in 3D spheroid culture.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-05T14:39:13.166Z","creation":"2025-04-05T14:39:13.166Z"},"accession":"S-EPMC10939856","cross_references":{"pubmed":["38142299"],"doi":["10.1002/adhm.202302528"]}}