{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou B"],"funding":["NSF | BIO | Division of Molecular and Cellular Biosciences","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)","NSF | BIO | Division of Molecular and Cellular Biosciences (MCB)"],"pagination":["2399"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10944506"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["MicroRNAs (miRNAs) play a key role in regulating gene expression and their biogenesis is precisely controlled through modulating the activity of microprocessor. Here, we report that CWC15, a spliceosome-associated protein, acts as a positive regulator of miRNA biogenesis. CWC15 binds the promoters of genes encoding miRNAs (MIRs), promotes their activity, and increases the occupancy of DNA-dependent RNA polymerases at MIR promoters, suggesting that CWC15 positively regulates the transcription of primary miRNA transcripts (pri-miRNAs). In addition, CWC15 interacts with Serrate (SE) and HYL1, two key components of microprocessor, and is required for efficient pri-miRNA processing and the HYL1-pri-miRNA interaction. Moreover, CWC15 interacts with the 20 S proteasome and PRP4KA, facilitating SE phosphorylation by PRP4KA, and subsequent non-functional SE degradation by the 20 S proteasome. These data reveal that CWC15 ensures optimal miRNA biogenesis by maintaining proper SE levels and by modulating pri-miRNA levels. Taken together, this study uncovers the role of a conserved splicing-related protein in miRNA biogenesis."],"journal":["Nature communications"],"pubmed_title":["The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis."],"pmcid":["PMC10944506"],"funding_grant_id":["MCB-1818082","GM127414"],"pubmed_authors":["Yu B","Zhou B","Zhang C","Yu H","Li M","Xue Y"],"additional_accession":[]},"is_claimable":false,"name":"The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis.","description":"MicroRNAs (miRNAs) play a key role in regulating gene expression and their biogenesis is precisely controlled through modulating the activity of microprocessor. Here, we report that CWC15, a spliceosome-associated protein, acts as a positive regulator of miRNA biogenesis. CWC15 binds the promoters of genes encoding miRNAs (MIRs), promotes their activity, and increases the occupancy of DNA-dependent RNA polymerases at MIR promoters, suggesting that CWC15 positively regulates the transcription of primary miRNA transcripts (pri-miRNAs). In addition, CWC15 interacts with Serrate (SE) and HYL1, two key components of microprocessor, and is required for efficient pri-miRNA processing and the HYL1-pri-miRNA interaction. Moreover, CWC15 interacts with the 20 S proteasome and PRP4KA, facilitating SE phosphorylation by PRP4KA, and subsequent non-functional SE degradation by the 20 S proteasome. These data reveal that CWC15 ensures optimal miRNA biogenesis by maintaining proper SE levels and by modulating pri-miRNA levels. Taken together, this study uncovers the role of a conserved splicing-related protein in miRNA biogenesis.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-04-20T02:51:11.397Z","creation":"2025-04-20T02:51:11.397Z"},"accession":"S-EPMC10944506","cross_references":{"pubmed":["38493158"],"doi":["10.1038/s41467-024-46676-z"]}}