{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Damhorst GL"],"funding":["NCATS","NCATS NIH HHS","NIDCD","NIBIB"],"pagination":["e27188"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10945130"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(6)"],"pubmed_abstract":["Limited data highlight the need to understand differences in SARS-CoV-2 omicron (B.1.1.529) variant viral load between the gold standard nasopharyngeal (NP) swab, mid-turbinate (MT)/anterior nasal swabs, oropharyngeal (OP) swabs, and saliva. MT, OP, and saliva samples from symptomatic individuals in Atlanta, GA, in January 2022 and longitudinal samples from a small familial cohort were tested by both RT-PCR and ultrasensitive antigen assays. Higher concentrations in the nares were observed in the familial cohort, but a dominant sample type was not found among 39 cases in the cross-sectional cohort. The composite of positive MT or OP assay for both RT-PCR and antigen assay trended toward higher diagnostic yield but did not achieve significant difference. Our data did not identify a singular preferred sample type for SARS-CoV-2 testing, but higher levels of saliva nucleocapsid, a trend toward higher yield of composite OP/MT result, and association of apparent MT or OP predominance with symptoms warrant further study."],"journal":["Heliyon"],"pubmed_title":["Comparison of RT-PCR and antigen test sensitivity across nasopharyngeal, nares, and oropharyngeal swab, and saliva sample types during the SARS-CoV-2 omicron variant."],"pmcid":["PMC10945130"],"funding_grant_id":["UL1 TR002378"],"pubmed_authors":["Piantadosi AL","Greenleaf M","Pollock NR","Swanson S","Frediani JK","O'Neal JW","McLendon K","Martin GS","Lam WA","Roback JD","Levy JM","Waggoner JJ","Rao A","Baugh TJ","O'Sick WH","Lin J","Bassit L","Sullivan JA","Westbrook A","Damhorst GL"],"additional_accession":[]},"is_claimable":false,"name":"Comparison of RT-PCR and antigen test sensitivity across nasopharyngeal, nares, and oropharyngeal swab, and saliva sample types during the SARS-CoV-2 omicron variant.","description":"Limited data highlight the need to understand differences in SARS-CoV-2 omicron (B.1.1.529) variant viral load between the gold standard nasopharyngeal (NP) swab, mid-turbinate (MT)/anterior nasal swabs, oropharyngeal (OP) swabs, and saliva. MT, OP, and saliva samples from symptomatic individuals in Atlanta, GA, in January 2022 and longitudinal samples from a small familial cohort were tested by both RT-PCR and ultrasensitive antigen assays. Higher concentrations in the nares were observed in the familial cohort, but a dominant sample type was not found among 39 cases in the cross-sectional cohort. The composite of positive MT or OP assay for both RT-PCR and antigen assay trended toward higher diagnostic yield but did not achieve significant difference. Our data did not identify a singular preferred sample type for SARS-CoV-2 testing, but higher levels of saliva nucleocapsid, a trend toward higher yield of composite OP/MT result, and association of apparent MT or OP predominance with symptoms warrant further study.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-28T03:09:55.282Z","creation":"2026-06-28T03:06:49.747Z"},"accession":"S-EPMC10945130","cross_references":{"pubmed":["38500996"],"doi":["10.1016/j.heliyon.2024.e27188"]}}