{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Adilkhanova A"],"funding":["Collaborative Research Program, Nazarbayev University","Nottingham Ningbo China Beacons of Excellence Research and Innovation Institute"],"pagination":["e14426"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10945395"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(3)"],"pubmed_abstract":["Fluctuations in redox conditions in bioprocesses can alter the end-products, reduce their concentration, and lengthen the process time. Electrofermentation enables rapid metabolic modulation of biosynthesis and allows control of redox imbalances in biofilm-based fermentation processes. In this study, electrofermentation is used to boost the production of the bacterial biopolymer poly-γ-glutamic acid (γ-PGA) from Bacillus subtilis ATCC 6051. When compared to control experiments (3.3 ± 0.99 g L<sup>-1</sup> ), the application of an electrode potential E = 0.4 V versus Ag/AgCl results in a more than two-fold increase in the production of γ-PGA (9.13 ± 1.4 g L<sup>-1</sup> ). Using an engineered B. subtilis strain, in which γ-PGA production is driven by isopropyl β-d-1-thiogalactopyranoside, electrofermentation improves polymer concentrations from 15.4 ± 1.5 to 23.1 ± 1.6 versus g L<sup>-1</sup> . These results confirm that electrofermentation conditions can be adopted to increase the concentration of γ-PGA and perhaps other extracellular biopolymers in industrial strains."],"journal":["Microbial biotechnology"],"pubmed_title":["Electrofermentation increases concentration of poly γ-glutamic acid in Bacillus subtilis biofilms."],"pmcid":["PMC10945395"],"funding_grant_id":["021220CRP0522","I01220800007"],"pubmed_authors":["Adilkhanova A","Olaifa K","Abbas AH","Marsili E","Eghtesadi N","Ajunwa OM","Ormantayeva A","Calvio C","Kaziullayeva A","Pham TT"],"additional_accession":[]},"is_claimable":false,"name":"Electrofermentation increases concentration of poly γ-glutamic acid in Bacillus subtilis biofilms.","description":"Fluctuations in redox conditions in bioprocesses can alter the end-products, reduce their concentration, and lengthen the process time. Electrofermentation enables rapid metabolic modulation of biosynthesis and allows control of redox imbalances in biofilm-based fermentation processes. In this study, electrofermentation is used to boost the production of the bacterial biopolymer poly-γ-glutamic acid (γ-PGA) from Bacillus subtilis ATCC 6051. When compared to control experiments (3.3 ± 0.99 g L<sup>-1</sup> ), the application of an electrode potential E = 0.4 V versus Ag/AgCl results in a more than two-fold increase in the production of γ-PGA (9.13 ± 1.4 g L<sup>-1</sup> ). Using an engineered B. subtilis strain, in which γ-PGA production is driven by isopropyl β-d-1-thiogalactopyranoside, electrofermentation improves polymer concentrations from 15.4 ± 1.5 to 23.1 ± 1.6 versus g L<sup>-1</sup> . These results confirm that electrofermentation conditions can be adopted to increase the concentration of γ-PGA and perhaps other extracellular biopolymers in industrial strains.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2025-05-18T12:54:03.032Z","creation":"2025-05-18T12:54:03.032Z"},"accession":"S-EPMC10945395","cross_references":{"pubmed":["38497275"],"doi":["10.1111/1751-7915.14426"]}}