biostudies-literatureAdilkhanova ACollaborative Research Program, Nazarbayev UniversityNottingham Ningbo China Beacons of Excellence Research and Innovation Institutee14426https://www.ebi.ac.uk/biostudies/studies/S-EPMC10945395biostudies-literatureUnknown17(3)Fluctuations in redox conditions in bioprocesses can alter the end-products, reduce their concentration, and lengthen the process time. Electrofermentation enables rapid metabolic modulation of biosynthesis and allows control of redox imbalances in biofilm-based fermentation processes. In this study, electrofermentation is used to boost the production of the bacterial biopolymer poly-γ-glutamic acid (γ-PGA) from Bacillus subtilis ATCC 6051. When compared to control experiments (3.3 ± 0.99 g L^-1 ), the application of an electrode potential E = 0.4 V versus Ag/AgCl results in a more than two-fold increase in the production of γ-PGA (9.13 ± 1.4 g L^-1 ). Using an engineered B. subtilis strain, in which γ-PGA production is driven by isopropyl β-d-1-thiogalactopyranoside, electrofermentation improves polymer concentrations from 15.4 ± 1.5 to 23.1 ± 1.6 versus g L^-1 . These results confirm that electrofermentation conditions can be adopted to increase the concentration of γ-PGA and perhaps other extracellular biopolymers in industrial strains.Microbial biotechnologyElectrofermentation increases concentration of poly γ-glutamic acid in Bacillus subtilis biofilms.PMC10945395021220CRP0522I01220800007Adilkhanova AOlaifa KAbbas AHMarsili EEghtesadi NAjunwa OMOrmantayeva ACalvio CKaziullayeva APham TTfalseElectrofermentation increases concentration of poly γ-glutamic acid in Bacillus subtilis biofilms.Fluctuations in redox conditions in bioprocesses can alter the end-products, reduce their concentration, and lengthen the process time. Electrofermentation enables rapid metabolic modulation of biosynthesis and allows control of redox imbalances in biofilm-based fermentation processes. In this study, electrofermentation is used to boost the production of the bacterial biopolymer poly-γ-glutamic acid (γ-PGA) from Bacillus subtilis ATCC 6051. When compared to control experiments (3.3 ± 0.99 g L^-1 ), the application of an electrode potential E = 0.4 V versus Ag/AgCl results in a more than two-fold increase in the production of γ-PGA (9.13 ± 1.4 g L^-1 ). Using an engineered B. subtilis strain, in which γ-PGA production is driven by isopropyl β-d-1-thiogalactopyranoside, electrofermentation improves polymer concentrations from 15.4 ± 1.5 to 23.1 ± 1.6 versus g L^-1 . These results confirm that electrofermentation conditions can be adopted to increase the concentration of γ-PGA and perhaps other extracellular biopolymers in industrial strains.2024-01-01T00:00:00Z2024 Mar2025-05-18T12:54:03.032Z2025-05-18T12:54:03.032ZS-EPMC109453953849727510.1111/1751-7915.14426