{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["121(11)"],"submitter":["Francia V"],"pubmed_abstract":["Lipid nanoparticle (LNP) formulations are a proven method for the delivery of nucleic acids for gene therapy as exemplified by the worldwide rollout of LNP-based RNAi therapeutics and mRNA vaccines. However, targeting specific tissues or cells is still a major challenge. After LNP administration, LNPs interact with biological fluids (i.e., blood), components of which adsorb onto the LNP surface forming a layer of biomolecules termed the \"biomolecular corona (BMC)\" which affects LNP stability, biodistribution, and tissue tropism. The mechanisms by which the BMC influences tissue- and cell-specific targeting remains largely unknown, due to the technical challenges in isolating LNPs and their corona from complex biological media. In this study, we present a new technique that utilizes magneti"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["e2307803120"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10945860"],"repository":["biostudies-literature"],"pubmed_title":["A magnetic separation method for isolating and characterizing the biomolecular corona of lipid nanoparticles."],"pmcid":["PMC10945860"],"pubmed_authors":["Zhang Y","Witzigmann D","Francia V","Schiffelers RM","Cullis PR","Cheng MHY"],"additional_accession":[]},"is_claimable":false,"name":"A magnetic separation method for isolating and characterizing the biomolecular corona of lipid nanoparticles.","description":"Lipid nanoparticle (LNP) formulations are a proven method for the delivery of nucleic acids for gene therapy as exemplified by the worldwide rollout of LNP-based RNAi therapeutics and mRNA vaccines. However, targeting specific tissues or cells is still a major challenge. After LNP administration, LNPs interact with biological fluids (i.e., blood), components of which adsorb onto the LNP surface forming a layer of biomolecules termed the \"biomolecular corona (BMC)\" which affects LNP stability, biodistribution, and tissue tropism. The mechanisms by which the BMC influences tissue- and cell-specific targeting remains largely unknown, due to the technical challenges in isolating LNPs and their corona from complex biological media. In this study, we present a new technique that utilizes magneti","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-07-14T22:48:29.394Z","creation":"2025-02-19T03:09:09.561Z"},"accession":"S-EPMC10945860","cross_references":{"pubmed":["38437542"],"doi":["10.1073/pnas.2307803120"]}}