{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["133(8)"],"submitter":["Sappakhaw K"],"pubmed_abstract":["Polypeptides generated from proteolytic processing of protein precursors, or proteolytic proteoforms, play an important role in diverse biological functions and diseases. However, their often-small size and intricate post-translational biogenesis preclude the use of simple genetic tagging in their cellular studies. Herein, we develop a labeling strategy for this class of proteoforms, based on residue-specific genetic code expansion labeling with a molecular beacon design. We demonstrate the utility of such a design by creating a molecular beacon reporter to detect amyloid-β peptides, known to be involved in the pathogenesis of Alzheimer's disease, as they are produced from amyloid precursor protein (APP) along the endocytic pathway of living cells."],"journal":["Angewandte Chemie (Weinheim an der Bergstrasse, Germany)"],"pagination":["3980-3985"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10946459"],"repository":["biostudies-literature"],"pubmed_title":["A Genetic Code Expansion-Derived Molecular Beacon for the Detection of Intracellular Amyloid-β Peptide Generation."],"pmcid":["PMC10946459"],"pubmed_authors":["Slavoff SA","Sappakhaw K","Jantarug K","Uttamapinant C","Israsena N"],"additional_accession":[]},"is_claimable":false,"name":"A Genetic Code Expansion-Derived Molecular Beacon for the Detection of Intracellular Amyloid-β Peptide Generation.","description":"Polypeptides generated from proteolytic processing of protein precursors, or proteolytic proteoforms, play an important role in diverse biological functions and diseases. However, their often-small size and intricate post-translational biogenesis preclude the use of simple genetic tagging in their cellular studies. Herein, we develop a labeling strategy for this class of proteoforms, based on residue-specific genetic code expansion labeling with a molecular beacon design. We demonstrate the utility of such a design by creating a molecular beacon reporter to detect amyloid-β peptides, known to be involved in the pathogenesis of Alzheimer's disease, as they are produced from amyloid precursor protein (APP) along the endocytic pathway of living cells.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2025-04-26T12:06:51.742Z","creation":"2025-02-19T03:08:57.829Z"},"accession":"S-EPMC10946459","cross_references":{"pubmed":["38504667"],"doi":["10.1002/ange.202010703"]}}