biostudies-literatureUnknown133(19)Anbu SManganese-based contrast agents (MnCAs) have emerged as suitable alternatives to gadolinium-based contrast agents (GdCAs). However, due to their kinetic lability and laborious synthetic procedures, only a few MnCAs have found clinical MRI application. In this work, we have employed a highly innovative single-pot template synthetic strategy to develop a MnCA, <b>MnL^Me</b>, and studied the most important physicochemical properties in vitro. <b>MnL^Me</b> displays optimized <i>r</i> ₁ relaxivities at both medium (20 and 64 MHz) and high magnetic fields (300 and 400 MHz) and an enhanced <i>r</i> ₁ ^b=21.1 mM^-1 s^-1 (20 MHz, 298 K, pH 7.4) upon binding to BSA (<i>K</i> _a=4.2×10³ M^-1). In vAngewandte Chemie (Weinheim an der Bergstrasse, Germany)10831-10839https://www.ebi.ac.uk/biostudies/studies/S-EPMC10947048biostudies-literatureA Single-Pot Template Reaction Towards a Manganese-Based <i>T</i>₁ Contrast Agent.PMC10947048Martins AFAnbu SHoffmann SHLKenning LCarniato FPrice TWStasiuk GJPrior TJBotta MfalseA Single-Pot Template Reaction Towards a Manganese-Based <i>T</i>₁ Contrast Agent.Manganese-based contrast agents (MnCAs) have emerged as suitable alternatives to gadolinium-based contrast agents (GdCAs). However, due to their kinetic lability and laborious synthetic procedures, only a few MnCAs have found clinical MRI application. In this work, we have employed a highly innovative single-pot template synthetic strategy to develop a MnCA, <b>MnL^Me</b>, and studied the most important physicochemical properties in vitro. <b>MnL^Me</b> displays optimized <i>r</i> ₁ relaxivities at both medium (20 and 64 MHz) and high magnetic fields (300 and 400 MHz) and an enhanced <i>r</i> ₁ ^b=21.1 mM^-1 s^-1 (20 MHz, 298 K, pH 7.4) upon binding to BSA (<i>K</i> _a=4.2×10³ M^-1). In v2021-01-01T00:00:00Z2021 May2025-04-26T12:14:30.091Z2025-04-06T13:53:57.884ZS-EPMC109470483850569010.1002/ange.202100885