{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["133(34)"],"submitter":["Mattey AP"],"pubmed_abstract":["A key aim of biocatalysis is to mimic the ability of eukaryotic cells to carry out multistep cascades in a controlled and selective way. As biocatalytic cascades get more complex, reactions become unattainable under typical batch conditions. Here a number of continuous flow systems were used to overcome batch incompatibility, thus allowing for successful biocatalytic cascades. As proof-of-principle, reactive carbonyl intermediates were generated in situ using alcohol oxidases, then passed directly to a series of packed-bed modules containing different aminating biocatalysts which accordingly produced a range of structurally distinct amines. The method was expanded to employ a batch incompatible sequential amination cascade via an oxidase/transaminase/imine reductase sequence, introducing different amine reagents at each step without cross-reactivity. The combined approaches allowed for the biocatalytic synthesis of the natural product 4O-methylnorbelladine."],"journal":["Angewandte Chemie (Weinheim an der Bergstrasse, Germany)"],"pagination":["18808-18813"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10947180"],"repository":["biostudies-literature"],"pubmed_title":["Development of Continuous Flow Systems to Access Secondary Amines Through Previously Incompatible Biocatalytic Cascades."],"pmcid":["PMC10947180"],"pubmed_authors":["Citoler J","Flitsch SL","Baldwin C","Cosgrove SC","Mattey AP","Marshall JR","Thompson M","Palmer RB","Ford GJ","Turner NJ"],"additional_accession":[]},"is_claimable":false,"name":"Development of Continuous Flow Systems to Access Secondary Amines Through Previously Incompatible Biocatalytic Cascades.","description":"A key aim of biocatalysis is to mimic the ability of eukaryotic cells to carry out multistep cascades in a controlled and selective way. As biocatalytic cascades get more complex, reactions become unattainable under typical batch conditions. Here a number of continuous flow systems were used to overcome batch incompatibility, thus allowing for successful biocatalytic cascades. As proof-of-principle, reactive carbonyl intermediates were generated in situ using alcohol oxidases, then passed directly to a series of packed-bed modules containing different aminating biocatalysts which accordingly produced a range of structurally distinct amines. The method was expanded to employ a batch incompatible sequential amination cascade via an oxidase/transaminase/imine reductase sequence, introducing different amine reagents at each step without cross-reactivity. The combined approaches allowed for the biocatalytic synthesis of the natural product 4O-methylnorbelladine.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Aug","modification":"2025-04-26T12:15:23.61Z","creation":"2025-04-06T13:54:08.982Z"},"accession":"S-EPMC10947180","cross_references":{"pubmed":["38505092"],"doi":["10.1002/ange.202103805"]}}