biostudies-literatureRooney CHEBritish Heart FoundationIsrael Science FoundationEuropean Commissione202300144https://www.ebi.ac.uk/biostudies/studies/S-EPMC10947409biostudies-literatureUnknown24(18)Deuterated ¹³ C sites in sugars (D-glucose and 2-deoxy-D-glucose) showed 6.3-to-17.5-fold higher solid-state dynamic nuclear polarization (DNP) levels than their respective protonated sites at 3.35T. This effect was found to be unrelated to the protonation of the bath. Deuterated ¹⁵ N in sites bound to exchangeable protons ([¹⁵ N₂ ]urea) showed a 1.3-fold higher polarization than their respective protonated sites at the same magnetic field. This relatively smaller effect was attributed to incomplete deuteration of the ¹⁵ N sites due to the solvent mixture. For a ¹⁵ N site that is not bound to protons or deuterons ([¹⁵ N]nitrate), deuteration of the bath did not affect the polarization level. These findings suggest a phenomenon related to DNP of X-nuclei directly bound to deuteron(s) as opposed to proton(s). It appears that direct binding to deuterons increases the solid-state DNP polarization level of X-nuclei which are otherwise bound to protons.Chemphyschem : a European journal of chemical physics and physical chemistryDirectly Bound Deuterons Increase X-Nuclei Hyperpolarization using Dynamic Nuclear Polarization.PMC10947409FS/19/18/342521379/18858149Tyler DJRooney CHEKatz-Brull RGrist JTShaul DGamliel AfalseDirectly Bound Deuterons Increase X-Nuclei Hyperpolarization using Dynamic Nuclear Polarization.Deuterated ¹³ C sites in sugars (D-glucose and 2-deoxy-D-glucose) showed 6.3-to-17.5-fold higher solid-state dynamic nuclear polarization (DNP) levels than their respective protonated sites at 3.35T. This effect was found to be unrelated to the protonation of the bath. Deuterated ¹⁵ N in sites bound to exchangeable protons ([¹⁵ N₂ ]urea) showed a 1.3-fold higher polarization than their respective protonated sites at the same magnetic field. This relatively smaller effect was attributed to incomplete deuteration of the ¹⁵ N sites due to the solvent mixture. For a ¹⁵ N site that is not bound to protons or deuterons ([¹⁵ N]nitrate), deuteration of the bath did not affect the polarization level. These findings suggest a phenomenon related to DNP of X-nuclei directly bound to deuteron(s) as opposed to proton(s). It appears that direct binding to deuterons increases the solid-state DNP polarization level of X-nuclei which are otherwise bound to protons.2023-01-01T00:00:00Z2023 Sep2025-04-20T02:49:43.468Z2025-02-19T03:09:12.823ZS-EPMC109474093743162210.1002/cphc.202300144