{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hupfeld KE"],"funding":["NIBIB NIH HHS","National Institute on Alcohol Abuse and Alcoholism","NIDA NIH HHS","NIA NIH HHS","NIAAA NIH HHS","National Institute on Drug Abuse","National Institute of Biomedical Imaging and Bioengineering","National Institute on Aging"],"pagination":["e5076"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10947947"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["37(4)"],"pubmed_abstract":["Literature values vary widely for within-subject test-retest reproducibility of gamma-aminobutyric acid (GABA) measured with edited magnetic resonance spectroscopy (MRS). Reasons for this variation remain unclear. Here, we tested whether three acquisition parameters-(1) sequence complexity (two-experiment MEscher-GArwood Point RESolved Spectroscopy [MEGA-PRESS] vs. four-experiment Hadamard Encoding and Reconstruction of MEGA-Edited Spectroscopy [HERMES]); (2) editing pulse duration (14 vs. 20 ms); and (3) scanner frequency drift (interleaved water referencing [IWR] turned ON vs. OFF)-and two linear combination modeling variations-(1) three different coedited macromolecule models (called \"1to1GABA\", \"1to1GABAsoft\", and \"3to2MM\" in the Osprey software package); and (2) 0.55- versus 0.4-ppm spline baseline knot spacing-affected the within-subject coefficient of variation of GABA + macromolecules (GABA+). We collected edited MRS data from the dorsal anterior cingulate cortex from 20 participants (mean age: 30.8 ± 9.5 years; 10 males). Test and retest scans were separated by removing the participant from the scanner for 5-10 min. Each acquisition consisted of two MEGA-PRESS and two HERMES sequences with editing pulse durations of 14 and 20 ms (referred to here as MEGA-14, MEGA-20, HERMES-14, and HERMES-20; all TE = 80 ms, 224 averages). We identified the best test-retest reproducibility following postprocessing with a composite model of the 0.9- and 3-ppm macromolecules (\"3to2MM\"); this model performed particularly well for the HERMES data. Furthermore, sparser (0.55- compared with 0.4-ppm) spline baseline knot spacing yielded generally better test-retest reproducibility for GABA+. Replicating our prior results, linear combination modeling in Osprey compared with simple peak fitting in Gannet resulted in substantially better test-retest reproducibility. However, reproducibility did not consistently differ for MEGA-PRESS compared with HERMES, for 14- compared with 20-ms editing pulses, or for IWR-ON versus IWR-OFF. These results highlight the importance of model selection for edited MRS studies of GABA+, particularly for clinical studies that focus on individual patient differences in GABA+ or changes following an intervention."],"journal":["NMR in biomedicine"],"pubmed_title":["Impact of acquisition and modeling parameters on the test-retest reproducibility of edited GABA."],"pmcid":["PMC10947947"],"funding_grant_id":["K00AG068440","K24 AA030788","R01 AA025365","K99 AG080084","K99AG080084","P50 AA010761","K00 AG068440","R21 EB033516","P41 EB031771","R00 AG062230","R01 EB023963","R01 DA054275"],"pubmed_authors":["Murali-Manohar S","Hui SCN","Hupfeld KE","Yedavalli V","Prisciandaro JJ","Edden RAE","Zollner HJ","Oeltzschner G","Song Y"],"additional_accession":[]},"is_claimable":false,"name":"Impact of acquisition and modeling parameters on the test-retest reproducibility of edited GABA.","description":"Literature values vary widely for within-subject test-retest reproducibility of gamma-aminobutyric acid (GABA) measured with edited magnetic resonance spectroscopy (MRS). Reasons for this variation remain unclear. Here, we tested whether three acquisition parameters-(1) sequence complexity (two-experiment MEscher-GArwood Point RESolved Spectroscopy [MEGA-PRESS] vs. four-experiment Hadamard Encoding and Reconstruction of MEGA-Edited Spectroscopy [HERMES]); (2) editing pulse duration (14 vs. 20 ms); and (3) scanner frequency drift (interleaved water referencing [IWR] turned ON vs. OFF)-and two linear combination modeling variations-(1) three different coedited macromolecule models (called \"1to1GABA\", \"1to1GABAsoft\", and \"3to2MM\" in the Osprey software package); and (2) 0.55- versus 0.4-ppm spline baseline knot spacing-affected the within-subject coefficient of variation of GABA + macromolecules (GABA+). We collected edited MRS data from the dorsal anterior cingulate cortex from 20 participants (mean age: 30.8 ± 9.5 years; 10 males). Test and retest scans were separated by removing the participant from the scanner for 5-10 min. Each acquisition consisted of two MEGA-PRESS and two HERMES sequences with editing pulse durations of 14 and 20 ms (referred to here as MEGA-14, MEGA-20, HERMES-14, and HERMES-20; all TE = 80 ms, 224 averages). We identified the best test-retest reproducibility following postprocessing with a composite model of the 0.9- and 3-ppm macromolecules (\"3to2MM\"); this model performed particularly well for the HERMES data. Furthermore, sparser (0.55- compared with 0.4-ppm) spline baseline knot spacing yielded generally better test-retest reproducibility for GABA+. Replicating our prior results, linear combination modeling in Osprey compared with simple peak fitting in Gannet resulted in substantially better test-retest reproducibility. However, reproducibility did not consistently differ for MEGA-PRESS compared with HERMES, for 14- compared with 20-ms editing pulses, or for IWR-ON versus IWR-OFF. These results highlight the importance of model selection for edited MRS studies of GABA+, particularly for clinical studies that focus on individual patient differences in GABA+ or changes following an intervention.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2025-07-02T03:04:12.745Z","creation":"2025-07-02T03:04:12.745Z"},"accession":"S-EPMC10947947","cross_references":{"pubmed":["38091628"],"doi":["10.1002/nbm.5076"]}}