{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Orford K"],"funding":["NIDDK NIH HHS","NIGMS NIH HHS"],"pagination":["798-809"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10948009"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(5)"],"pubmed_abstract":["Throughout development, cell fate decisions are converted into epigenetic information that determines cellular identity. Covalent histone modifications are heritable epigenetic marks and are hypothesized to play a central role in this process. In this report, we assess the concordance of histone H3 lysine 4 dimethylation (H3K4me2) and trimethylation (H3K4me3) on a genome-wide scale in erythroid development by analyzing pluripotent, multipotent, and unipotent cell types. Although H3K4me2 and H3K4me3 are concordant at most genes, multipotential hematopoietic cells have a subset of genes that are differentially methylated (H3K4me2+/me3-). These genes are transcriptionally silent, highly enriched in lineage-specific hematopoietic genes, and uniquely susceptible to differentiation-induced H3K4 demethylation. Self-renewing embryonic stem cells, which restrict H3K4 methylation to genes that contain CpG islands (CGIs), lack H3K4me2+/me3- genes. These data reveal distinct epigenetic regulation of CGI and non-CGI genes during development and indicate an interactive relationship between DNA sequence and differential H3K4 methylation in lineage-specific differentiation."],"journal":["Developmental cell"],"pubmed_title":["Differential H3K4 methylation identifies developmentally poised hematopoietic genes."],"pmcid":["PMC10948009"],"funding_grant_id":["K08 DK070754","K25 GM067825"],"pubmed_authors":["Park PJ","Worhunsky DJ","Kharchenko P","Lai W","Dao MC","Ferro A","Scadden DT","Orford K","Janzen V"],"additional_accession":[]},"is_claimable":false,"name":"Differential H3K4 methylation identifies developmentally poised hematopoietic genes.","description":"Throughout development, cell fate decisions are converted into epigenetic information that determines cellular identity. Covalent histone modifications are heritable epigenetic marks and are hypothesized to play a central role in this process. In this report, we assess the concordance of histone H3 lysine 4 dimethylation (H3K4me2) and trimethylation (H3K4me3) on a genome-wide scale in erythroid development by analyzing pluripotent, multipotent, and unipotent cell types. Although H3K4me2 and H3K4me3 are concordant at most genes, multipotential hematopoietic cells have a subset of genes that are differentially methylated (H3K4me2+/me3-). These genes are transcriptionally silent, highly enriched in lineage-specific hematopoietic genes, and uniquely susceptible to differentiation-induced H3K4 demethylation. Self-renewing embryonic stem cells, which restrict H3K4 methylation to genes that contain CpG islands (CGIs), lack H3K4me2+/me3- genes. These data reveal distinct epigenetic regulation of CGI and non-CGI genes during development and indicate an interactive relationship between DNA sequence and differential H3K4 methylation in lineage-specific differentiation.","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 May","modification":"2026-05-29T11:42:10.281Z","creation":"2026-04-08T04:31:51.631Z"},"accession":"S-EPMC10948009","cross_references":{"pubmed":["18477461"],"doi":["10.1016/j.devcel.2008.04.002"]}}