{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xiao T"],"funding":["American Heart Association","National Center for Research Resources","NIA NIH HHS","NCRR NIH HHS","American Cancer Society/Live Mass Southern Multifoods Postdoctoral","University of Utah","National Institutes of Health","NIH HHS","NIGMS NIH HHS"],"pagination":["e202302069"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10949074"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["223(5)"],"pubmed_abstract":["Cells utilize multiple mechanisms to maintain mitochondrial homeostasis. We recently characterized a pathway that remodels mitochondria in response to metabolic alterations and protein overload stress. This remodeling occurs via the formation of large membranous structures from the mitochondrial outer membrane called mitochondrial-derived compartments (MDCs), which are eventually released from mitochondria and degraded. Here, we conducted a microscopy-based screen in budding yeast to identify factors that regulate MDC formation. We found that two phospholipids, cardiolipin (CL) and phosphatidylethanolamine (PE), differentially regulate MDC biogenesis. CL depletion impairs MDC biogenesis, whereas blocking mitochondrial PE production leads to constitutive MDC formation. Additionally, in response to metabolic MDC activators, cellular and mitochondrial PE declines, and overexpressing mitochondrial PE synthesis enzymes suppress MDC biogenesis. Altogether, our data indicate a requirement for CL in MDC biogenesis and suggest that PE depletion may stimulate MDC formation downstream of MDC-inducing metabolic stress."],"journal":["The Journal of cell biology"],"pubmed_title":["The phospholipids cardiolipin and phosphatidylethanolamine differentially regulate MDC biogenesis."],"pmcid":["PMC10949074"],"funding_grant_id":["1S10OD021505-01","GM119694","1S10OD016232-01","20POST35200110","AG061376","S10 OD016232","S10 OD018210","T32 GM007464","T32GM007464","S10 OD021505","PF-20-018-01-CCG","R35 GM119694","1S10OD018210-01A1","R01 AG061376"],"pubmed_authors":["Maschek JA","English AM","Wilson ZN","Cox JE","Hughes AL","Xiao T"],"additional_accession":[]},"is_claimable":false,"name":"The phospholipids cardiolipin and phosphatidylethanolamine differentially regulate MDC biogenesis.","description":"Cells utilize multiple mechanisms to maintain mitochondrial homeostasis. We recently characterized a pathway that remodels mitochondria in response to metabolic alterations and protein overload stress. This remodeling occurs via the formation of large membranous structures from the mitochondrial outer membrane called mitochondrial-derived compartments (MDCs), which are eventually released from mitochondria and degraded. Here, we conducted a microscopy-based screen in budding yeast to identify factors that regulate MDC formation. We found that two phospholipids, cardiolipin (CL) and phosphatidylethanolamine (PE), differentially regulate MDC biogenesis. CL depletion impairs MDC biogenesis, whereas blocking mitochondrial PE production leads to constitutive MDC formation. Additionally, in response to metabolic MDC activators, cellular and mitochondrial PE declines, and overexpressing mitochondrial PE synthesis enzymes suppress MDC biogenesis. Altogether, our data indicate a requirement for CL in MDC biogenesis and suggest that PE depletion may stimulate MDC formation downstream of MDC-inducing metabolic stress.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-01T22:17:28.698Z","creation":"2025-04-06T09:32:00.732Z"},"accession":"S-EPMC10949074","cross_references":{"pubmed":["38497895"],"doi":["10.1083/jcb.202302069"]}}