biostudies-literatureHu RNatural Science Foundation of Liaoning ProvinceNational Natural Science Foundation of China48https://www.ebi.ac.uk/biostudies/studies/S-EPMC10949598biostudies-literatureUnknown26(1)<h4>Background</h4>Breast cancer stem cell (CSC) expansion results in tumor progression and chemoresistance; however, the modulation of CSC pluripotency remains unexplored. Transmembrane protein 120B (TMEM120B) is a newly discovered protein expressed in human tissues, especially in malignant tissues; however, its role in CSC expansion has not been studied. This study aimed to determine the role of TMEM120B in transcriptional coactivator with PDZ-binding motif (TAZ)-mediated CSC expansion and chemotherapy resistance.<h4>Methods</h4>Both bioinformatics analysis and immunohistochemistry assays were performed to examine expression patterns of TMEM120B in lung, breast, gastric, colon, and ovarian cancers. Clinicopathological factors and overall survival were also evaluated. Next, colony formatiBreast cancer research : BCRTMEM120B strengthens breast cancer cell stemness and accelerates chemotherapy resistance via β1-integrin/FAK-TAZ-mTOR signaling axis by binding to MYH9.PMC10949598820028902021-MS-1632020- MS-1832020-MS-173Deng NYang KFan MMa XYing JZhao TSun XHou YGuan MZhang YCao YZhang XWang YHu RfalseTMEM120B strengthens breast cancer cell stemness and accelerates chemotherapy resistance via β1-integrin/FAK-TAZ-mTOR signaling axis by binding to MYH9.<h4>Background</h4>Breast cancer stem cell (CSC) expansion results in tumor progression and chemoresistance; however, the modulation of CSC pluripotency remains unexplored. Transmembrane protein 120B (TMEM120B) is a newly discovered protein expressed in human tissues, especially in malignant tissues; however, its role in CSC expansion has not been studied. This study aimed to determine the role of TMEM120B in transcriptional coactivator with PDZ-binding motif (TAZ)-mediated CSC expansion and chemotherapy resistance.<h4>Methods</h4>Both bioinformatics analysis and immunohistochemistry assays were performed to examine expression patterns of TMEM120B in lung, breast, gastric, colon, and ovarian cancers. Clinicopathological factors and overall survival were also evaluated. Next, colony formati2024-01-01T00:00:00Z2024 Mar2026-06-01T17:24:12.078Z2025-04-06T07:45:26.224ZS-EPMC109495983850437410.1186/s13058-024-01802-z