biostudies-literatureCordero Padilla KNIAAA NIH HHSNIHNIGMS NIH HHSe0298966https://www.ebi.ac.uk/biostudies/studies/S-EPMC10950231biostudies-literatureUnknown19(3)Research has identified the large conductance voltage- and calcium-activated potassium channel (BK) as a key regulator of neuronal excitability genetically associated to behavioral alcohol tolerance. Sensitivity to ethanol at the molecular level is characterized by acute potentiation of channel activity. BK isoforms show variations in alcohol sensitivity and are differentially distributed on the plasma membrane surface in response to prolonged exposure. MicroRNA (MiRNA) targeting of alcohol-sensitive isoforms coupled with active internalization of BK channels in response to ethanol are believed to be key in establishing homeostatic adaptations that produce persistent changes within the plasma membrane of neurons. In fact, microRNA 9 (miR-9) upregulated expression is a key event in persistePloS oneBK ZERO isoform HEK293 stably transfected cell lines differing 3'UTRs to assess miR-9 regulation.PMC10950231NIH R01AA027808R01 AA027808P20 GM103642P30 GM1493672P20GM103642-08Guevarez Galan AMonefeldt GAMarrero HGVelazquez-Marrero CCordero Padilla KLloret-Torres MEfalseBK ZERO isoform HEK293 stably transfected cell lines differing 3'UTRs to assess miR-9 regulation.Research has identified the large conductance voltage- and calcium-activated potassium channel (BK) as a key regulator of neuronal excitability genetically associated to behavioral alcohol tolerance. Sensitivity to ethanol at the molecular level is characterized by acute potentiation of channel activity. BK isoforms show variations in alcohol sensitivity and are differentially distributed on the plasma membrane surface in response to prolonged exposure. MicroRNA (MiRNA) targeting of alcohol-sensitive isoforms coupled with active internalization of BK channels in response to ethanol are believed to be key in establishing homeostatic adaptations that produce persistent changes within the plasma membrane of neurons. In fact, microRNA 9 (miR-9) upregulated expression is a key event in persiste2024-01-01T00:00:00Z20242026-07-15T10:40:56.288Z2026-07-03T03:13:50.147ZS-EPMC109502313850267310.1371/journal.pone.0298966