{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tisdale RK"],"funding":["NHLBI NIH HHS","National Institutes of Health","NIH HHS"],"pagination":["e14037"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10950840"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(3)"],"pubmed_abstract":["Aversive symptoms, including insomnia experienced during opioid withdrawal, are a major drive to relapse; however, withdrawal-associated sleep symptomatology has been little explored in preclinical models. We describe here a model of opioid withdrawal in mice that resembles the sleep phenotype characteristic of withdrawal in humans. Male and female C57BL/6 mice were instrumented with telemeters to record electroencephalogram, electromyogram, activity and subcutaneous temperature. All mice received two treatments separated by a 16-day washout period: (1) saline (volume: 10 ml kg<sup>-1</sup>); or (2) ascending doses of morphine (5, 10, 20, 40 and 80 mg kg<sup>-1</sup>; volume: 10 ml kg<sup>-1</sup>) for 5 days at Zeitgeber time 1 and Zeitgeber time 13. Recordings for the first 71 hr after treatment discontinuation (withdrawal days 1-3) and for 24 hr on withdrawal days 5 and 7 were scored for sleep/wake state, and sleep architecture and electroencephalogram spectral data were analysed. Morphine was acutely wake- and activity-promoting, and non-rapid eye movement and rapid eye movement sleep were increased during the dark phase on withdrawal day 2 in both sexes. While non-rapid eye movement delta power (0.5-4.0 Hz), a measure of sleep intensity, was reduced during the light phase on withdrawal day 1 and the dark phase on withdrawal day 2 in both sexes, female mice also exhibited changes in the duration and the number of bouts of sleep/wake states. These observations of fragmented sleep on withdrawal days 1-3 suggest poorer sleep consolidation and a more pronounced withdrawal-associated sleep phenotype in female than in male mice. These data may indicate a greater sensitivity to morphine, a more distinct aversive sleep phenotype and/or a faster escalation to dependence in female mice."],"journal":["Journal of sleep research"],"pubmed_title":["Biological sex influences sleep phenotype in mice experiencing spontaneous opioid withdrawal."],"pmcid":["PMC10950840"],"funding_grant_id":["R01 HL150836","R01HL150836"],"pubmed_authors":["Allocca G","Tisdale RK","Haire M","Ma SC","Kilduff TS","Bruchas MR","Sun Y","Park S","Morairty SR"],"additional_accession":[]},"is_claimable":false,"name":"Biological sex influences sleep phenotype in mice experiencing spontaneous opioid withdrawal.","description":"Aversive symptoms, including insomnia experienced during opioid withdrawal, are a major drive to relapse; however, withdrawal-associated sleep symptomatology has been little explored in preclinical models. We describe here a model of opioid withdrawal in mice that resembles the sleep phenotype characteristic of withdrawal in humans. Male and female C57BL/6 mice were instrumented with telemeters to record electroencephalogram, electromyogram, activity and subcutaneous temperature. All mice received two treatments separated by a 16-day washout period: (1) saline (volume: 10 ml kg<sup>-1</sup>); or (2) ascending doses of morphine (5, 10, 20, 40 and 80 mg kg<sup>-1</sup>; volume: 10 ml kg<sup>-1</sup>) for 5 days at Zeitgeber time 1 and Zeitgeber time 13. Recordings for the first 71 hr after treatment discontinuation (withdrawal days 1-3) and for 24 hr on withdrawal days 5 and 7 were scored for sleep/wake state, and sleep architecture and electroencephalogram spectral data were analysed. Morphine was acutely wake- and activity-promoting, and non-rapid eye movement and rapid eye movement sleep were increased during the dark phase on withdrawal day 2 in both sexes. While non-rapid eye movement delta power (0.5-4.0 Hz), a measure of sleep intensity, was reduced during the light phase on withdrawal day 1 and the dark phase on withdrawal day 2 in both sexes, female mice also exhibited changes in the duration and the number of bouts of sleep/wake states. These observations of fragmented sleep on withdrawal days 1-3 suggest poorer sleep consolidation and a more pronounced withdrawal-associated sleep phenotype in female than in male mice. These data may indicate a greater sensitivity to morphine, a more distinct aversive sleep phenotype and/or a faster escalation to dependence in female mice.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2025-07-12T03:04:33.794Z","creation":"2025-07-12T03:04:33.794Z"},"accession":"S-EPMC10950840","cross_references":{"pubmed":["37731248"],"doi":["10.1111/jsr.14037"]}}