biostudies-literatureUnknown11(11)Ma JAO FoundationDiscogenic pain is associated with deep nerve ingrowth in annulus fibrosus tissue (AF) of intervertebral disc (IVD). To model AF nerve ingrowth, primary bovine dorsal root ganglion (DRG) micro-scale tissue units are spatially organised around an AF explant by mild hydrodynamic forces within a collagen matrix. This results in a densely packed multicellular system mimicking the native DRG tissue morphology and a controlled AF-neuron distance. Such a multicellular organisation is essential to evolve populational-level cellular functions and in vivo-like morphologies. Pro-inflammatory cytokine-primed AF demonstrates its neurotrophic and neurotropic effects on nociceptor axons. Both effects are dependent on the AF-neuron distance underpinning the role of recapitulating inter-tissue/organ anatomAdvanced science (Weinheim, Baden-Wurttemberg, Germany)e2308478https://www.ebi.ac.uk/biostudies/studies/S-EPMC10953573biostudies-literatureEngineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth.PMC10953573Eglauf JSerra TMa JGrad SAlini MfalseEngineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth.Discogenic pain is associated with deep nerve ingrowth in annulus fibrosus tissue (AF) of intervertebral disc (IVD). To model AF nerve ingrowth, primary bovine dorsal root ganglion (DRG) micro-scale tissue units are spatially organised around an AF explant by mild hydrodynamic forces within a collagen matrix. This results in a densely packed multicellular system mimicking the native DRG tissue morphology and a controlled AF-neuron distance. Such a multicellular organisation is essential to evolve populational-level cellular functions and in vivo-like morphologies. Pro-inflammatory cytokine-primed AF demonstrates its neurotrophic and neurotropic effects on nociceptor axons. Both effects are dependent on the AF-neuron distance underpinning the role of recapitulating inter-tissue/organ anatom2024-01-01T00:00:00Z2024 Mar2026-07-15T09:26:54.903Z2026-07-02T03:11:33.244ZS-EPMC109535733811331510.1002/advs.202308478