biostudies-literatureUnknown15Reinehr S<h4>Background</h4>The neurodegenerative processes leading to glaucoma are complex. In addition to elevated intraocular pressure (IOP), an involvement of immunological mechanisms is most likely. In the new multifactorial glaucoma model, a combination of high IOP and optic nerve antigen (ONA) immunization leads to an enhanced loss of retinal ganglion cells accompanied by a higher number of microglia/macrophages in the inner retina. Here, we aimed to evaluate the immune response in this new model, especially the complement activation and the number of T-cells, for the first time. Further, the microglia/macrophage response was examined in more detail.<h4>Methods</h4>Six-week-old wildtype (WT+ONA) and βB1-connective tissue growth factor high-pressure mice (CTGF+ONA) were immunized with 1 mg ONFrontiers in immunology1296178https://www.ebi.ac.uk/biostudies/studies/S-EPMC10955086biostudies-literatureIn a novel autoimmune and high-pressure glaucoma model a complex immune response is induced.PMC10955086Theile JDick HBSchulte KKJoachim SCWulf JFuchshofer RPeters MReinehr SfalseIn a novel autoimmune and high-pressure glaucoma model a complex immune response is induced.<h4>Background</h4>The neurodegenerative processes leading to glaucoma are complex. In addition to elevated intraocular pressure (IOP), an involvement of immunological mechanisms is most likely. In the new multifactorial glaucoma model, a combination of high IOP and optic nerve antigen (ONA) immunization leads to an enhanced loss of retinal ganglion cells accompanied by a higher number of microglia/macrophages in the inner retina. Here, we aimed to evaluate the immune response in this new model, especially the complement activation and the number of T-cells, for the first time. Further, the microglia/macrophage response was examined in more detail.<h4>Methods</h4>Six-week-old wildtype (WT+ONA) and βB1-connective tissue growth factor high-pressure mice (CTGF+ONA) were immunized with 1 mg ON2024-01-01T00:00:00Z20242025-04-19T05:48:48.623Z2024-11-13T04:47:09.705ZS-EPMC109550863851575510.3389/fimmu.2024.1296178