biostudies-literatureUnknown15Zhang JGuipi wan (GPW) is a traditional Chinese medicine commonly used in clinical practice, typically to treat neurological diseases such as neurasthenia and traumatic brain injury. It may have positive effects on cerebral ischemia‒reperfusion injury (cI/R). This study aimed to assess the effects of GPW in a mouse model of cI/R and find its possible targets. C57BL/6J mice were used to establish the cI/R model, and the laser speckle doppler was used to determine the success of the model. GPW was administered intragastrically for 7 days, brain tissue sections were stained with TTC, HE, and TUNEL, Western blot assay was performed to detect the effect of apoptosis-related proteins. Furthermore, we screened active ingredients from the TCM Database and constructed a compound‒target network using the CFrontiers in pharmacology1346226https://www.ebi.ac.uk/biostudies/studies/S-EPMC10955136biostudies-literaturePharmacological effects and target analysis of Guipi wan in the treatment of cerebral ischemia-reperfusion injury.PMC10955136Li XZhang JGuo YJiang WLuo LLiu ALiu QZhang MYu JfalsePharmacological effects and target analysis of Guipi wan in the treatment of cerebral ischemia-reperfusion injury.Guipi wan (GPW) is a traditional Chinese medicine commonly used in clinical practice, typically to treat neurological diseases such as neurasthenia and traumatic brain injury. It may have positive effects on cerebral ischemia‒reperfusion injury (cI/R). This study aimed to assess the effects of GPW in a mouse model of cI/R and find its possible targets. C57BL/6J mice were used to establish the cI/R model, and the laser speckle doppler was used to determine the success of the model. GPW was administered intragastrically for 7 days, brain tissue sections were stained with TTC, HE, and TUNEL, Western blot assay was performed to detect the effect of apoptosis-related proteins. Furthermore, we screened active ingredients from the TCM Database and constructed a compound‒target network using the C2024-01-01T00:00:00Z20242025-04-22T13:00:43.27Z2025-04-06T00:26:33.788ZS-EPMC109551363851583810.3389/fphar.2024.1346226