biostudies-literatureUnknown96(11)Eselem Bungu PSA new and robust high-performance liquid chromatography (HPLC) method that separates poly(lactic acid) (PLA) according to its stereochemical composition is presented. Using this method, poly(l-lactide) incorporating trace amounts of <i>meso</i>-lactide resulting from the racemization is separated from the pristine polymer. To prove this aspect in more detail, a representative poly(l-lactic acid) standard, assumed to be highly homogeneous, was separated using this method. The result showed that this was not the case as a fraction incorporating <i>meso</i>-lactide due to racemization occurring during the synthesis is separated. Employing two-dimensional liquid chromatography (2D-LC), the molar mass differences of the separated species were investigated, and fractions with similar molecular sAnalytical chemistry4716-4725https://www.ebi.ac.uk/biostudies/studies/S-EPMC10955512biostudies-literatureStereochemical Heterogeneity Analysis of Polylactides by Multidimensional Liquid Chromatography.PMC10955512Pasch HLuetzow KLettau OSchulz MNeffe ATEselem Bungu PSfalseStereochemical Heterogeneity Analysis of Polylactides by Multidimensional Liquid Chromatography.A new and robust high-performance liquid chromatography (HPLC) method that separates poly(lactic acid) (PLA) according to its stereochemical composition is presented. Using this method, poly(l-lactide) incorporating trace amounts of <i>meso</i>-lactide resulting from the racemization is separated from the pristine polymer. To prove this aspect in more detail, a representative poly(l-lactic acid) standard, assumed to be highly homogeneous, was separated using this method. The result showed that this was not the case as a fraction incorporating <i>meso</i>-lactide due to racemization occurring during the synthesis is separated. Employing two-dimensional liquid chromatography (2D-LC), the molar mass differences of the separated species were investigated, and fractions with similar molecular s2024-01-01T00:00:00Z2024 Mar2026-07-14T23:52:07.534Z2025-04-06T00:27:36.594ZS-EPMC109555123846544810.1021/acs.analchem.4c00336